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A Novel Zn Transporter Gene (ClZIP1) from Turmeric (Curcuma longa L.) and Expression Analysis in Presence of a Zn-Solubilizing Bacteria
Zinc (Zn) deficiency is widespread in plants and molecular mechanism of uptake and transport within organelles is unclear. A novel Zn transporter gene was identified and characterized from turmeric ( Curcuma longa L.) and expression analyzed in presence of zinc oxide (ZnO) and Bacillus safensis , a...
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| Published in: | Plant molecular biology reporter 2022-09, Vol.40 (3), p.500-515 |
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| creator | Sheeja, T. E. Praveena, R. Kumar, I. P. Vijesh Sarathambal, C. Shajina, O. Vijay, Hridya Rajeev, Nandana Mol, P. Prashina Sreena, C. P. Srinivasan, V. Dinesh, R. |
| description | Zinc (Zn) deficiency is widespread in plants and molecular mechanism of uptake and transport within organelles is unclear. A novel Zn transporter gene was identified and characterized from turmeric (
Curcuma longa
L.) and expression analyzed in presence of zinc oxide (ZnO) and
Bacillus safensis
, a Zn-solubilizing bacteria (ZSB). Initially, eleven Zn transporters were mined from rhizome-specific transcriptome of turmeric and the one with highest transcript abundance designated as
ClZIP1
was cloned and sequenced to give 1268-bp gene encoding 366 amino acids (ORF-1101 bp). In silico analysis and deduced protein designated ClZIP1 to Zn/Fe-regulated transporter (ZRT/IRT)-related protein (ZIP) family with 70.0% identity to Zn transporters from
Musa acuminata
. ClZIP1 possessed eight transmembrane (TM) domains with a variable region between TM-3 and TM-4 and conserved histidine-rich ZIP signature motif. A single 10-bp Zn deficiency-responsive element (ZDRE) was present in the promoter.
ClZIP1
expression evaluated in in vitro plantlets in presence of Zn applied as ZnO (10–100 ppm), with/without ZSB indicated that it was higher in basal portion than leaf. This is the first report on ZIP gene from turmeric, which showed higher expression in the absence of ZSB and vice versa with maximum downregulation at 100 ppm Zn (88%). |
| doi_str_mv | 10.1007/s11105-021-01317-3 |
| format | article |
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Curcuma longa
L.) and expression analyzed in presence of zinc oxide (ZnO) and
Bacillus safensis
, a Zn-solubilizing bacteria (ZSB). Initially, eleven Zn transporters were mined from rhizome-specific transcriptome of turmeric and the one with highest transcript abundance designated as
ClZIP1
was cloned and sequenced to give 1268-bp gene encoding 366 amino acids (ORF-1101 bp). In silico analysis and deduced protein designated ClZIP1 to Zn/Fe-regulated transporter (ZRT/IRT)-related protein (ZIP) family with 70.0% identity to Zn transporters from
Musa acuminata
. ClZIP1 possessed eight transmembrane (TM) domains with a variable region between TM-3 and TM-4 and conserved histidine-rich ZIP signature motif. A single 10-bp Zn deficiency-responsive element (ZDRE) was present in the promoter.
ClZIP1
expression evaluated in in vitro plantlets in presence of Zn applied as ZnO (10–100 ppm), with/without ZSB indicated that it was higher in basal portion than leaf. This is the first report on ZIP gene from turmeric, which showed higher expression in the absence of ZSB and vice versa with maximum downregulation at 100 ppm Zn (88%).</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-021-01317-3</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amino acids ; Aquatic plants ; Bacteria ; Bioinformatics ; Biomedical and Life Sciences ; Curcuma longa ; Histidine ; Life Sciences ; Metabolomics ; Nutrient deficiency ; Open reading frames ; Organelles ; Original Article ; Plant Breeding/Biotechnology ; Plant Sciences ; Plantlets ; Proteins ; Proteomics ; Regulatory sequences ; Rhizomes ; Transcriptomes ; Variable region ; Zinc oxide ; Zinc oxides</subject><ispartof>Plant molecular biology reporter, 2022-09, Vol.40 (3), p.500-515</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-c485ad666115a32028b0338a987f4d132c3b192fba867f15252cef80cdee58c13</citedby><cites>FETCH-LOGICAL-c319t-c485ad666115a32028b0338a987f4d132c3b192fba867f15252cef80cdee58c13</cites><orcidid>0000-0002-4020-4599</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sheeja, T. E.</creatorcontrib><creatorcontrib>Praveena, R.</creatorcontrib><creatorcontrib>Kumar, I. P. Vijesh</creatorcontrib><creatorcontrib>Sarathambal, C.</creatorcontrib><creatorcontrib>Shajina, O.</creatorcontrib><creatorcontrib>Vijay, Hridya</creatorcontrib><creatorcontrib>Rajeev, Nandana</creatorcontrib><creatorcontrib>Mol, P. Prashina</creatorcontrib><creatorcontrib>Sreena, C. P.</creatorcontrib><creatorcontrib>Srinivasan, V.</creatorcontrib><creatorcontrib>Dinesh, R.</creatorcontrib><title>A Novel Zn Transporter Gene (ClZIP1) from Turmeric (Curcuma longa L.) and Expression Analysis in Presence of a Zn-Solubilizing Bacteria</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>Zinc (Zn) deficiency is widespread in plants and molecular mechanism of uptake and transport within organelles is unclear. A novel Zn transporter gene was identified and characterized from turmeric (
Curcuma longa
L.) and expression analyzed in presence of zinc oxide (ZnO) and
Bacillus safensis
, a Zn-solubilizing bacteria (ZSB). Initially, eleven Zn transporters were mined from rhizome-specific transcriptome of turmeric and the one with highest transcript abundance designated as
ClZIP1
was cloned and sequenced to give 1268-bp gene encoding 366 amino acids (ORF-1101 bp). In silico analysis and deduced protein designated ClZIP1 to Zn/Fe-regulated transporter (ZRT/IRT)-related protein (ZIP) family with 70.0% identity to Zn transporters from
Musa acuminata
. ClZIP1 possessed eight transmembrane (TM) domains with a variable region between TM-3 and TM-4 and conserved histidine-rich ZIP signature motif. A single 10-bp Zn deficiency-responsive element (ZDRE) was present in the promoter.
ClZIP1
expression evaluated in in vitro plantlets in presence of Zn applied as ZnO (10–100 ppm), with/without ZSB indicated that it was higher in basal portion than leaf. This is the first report on ZIP gene from turmeric, which showed higher expression in the absence of ZSB and vice versa with maximum downregulation at 100 ppm Zn (88%).</description><subject>Amino acids</subject><subject>Aquatic plants</subject><subject>Bacteria</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Curcuma longa</subject><subject>Histidine</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Nutrient deficiency</subject><subject>Open reading frames</subject><subject>Organelles</subject><subject>Original Article</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Regulatory sequences</subject><subject>Rhizomes</subject><subject>Transcriptomes</subject><subject>Variable region</subject><subject>Zinc oxide</subject><subject>Zinc oxides</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kM1KAzEUhYMoWH9ewNUFN7pIzU06M5llLbUKRQXrxk1I00yJTJOadER9AV_baAV3ri4cznfO5RBygqyPjFUXCRFZQRlHylBgRcUO6WFRcVpLlLukxypR0LocsH1ykNIzyxCTskc-h3AbXm0LTx5mUfu0DnFjI0yst3A2ap9u7vEcmhhWMOviykZnstxF0600tMEvNUz756D9AsZv62hTcsHD0Ov2PbkEzsN9Fq03FkIDOtfQh9B2c9e6D-eXcKlNrnP6iOw1uk32-Pcekser8Wx0Tad3k5vRcEqNwHpDzUAWelGWJWKhBWdczpkQUteyagYLFNyIOda8mWtZVg0WvODGNpKZhbWFNCgOyek2dx3DS2fTRj2HLuZ3k-IVYikkF2V28a3LxJBStI1aR7fS8V0hU9-Dq-3gKg-ufgZXIkNiC6Vs9ksb_6L_ob4AC9qB5g</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Sheeja, T. E.</creator><creator>Praveena, R.</creator><creator>Kumar, I. P. Vijesh</creator><creator>Sarathambal, C.</creator><creator>Shajina, O.</creator><creator>Vijay, Hridya</creator><creator>Rajeev, Nandana</creator><creator>Mol, P. Prashina</creator><creator>Sreena, C. P.</creator><creator>Srinivasan, V.</creator><creator>Dinesh, R.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-4020-4599</orcidid></search><sort><creationdate>20220901</creationdate><title>A Novel Zn Transporter Gene (ClZIP1) from Turmeric (Curcuma longa L.) and Expression Analysis in Presence of a Zn-Solubilizing Bacteria</title><author>Sheeja, T. E. ; Praveena, R. ; Kumar, I. P. Vijesh ; Sarathambal, C. ; Shajina, O. ; Vijay, Hridya ; Rajeev, Nandana ; Mol, P. Prashina ; Sreena, C. 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P.</creatorcontrib><creatorcontrib>Srinivasan, V.</creatorcontrib><creatorcontrib>Dinesh, R.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheeja, T. E.</au><au>Praveena, R.</au><au>Kumar, I. P. Vijesh</au><au>Sarathambal, C.</au><au>Shajina, O.</au><au>Vijay, Hridya</au><au>Rajeev, Nandana</au><au>Mol, P. Prashina</au><au>Sreena, C. P.</au><au>Srinivasan, V.</au><au>Dinesh, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Zn Transporter Gene (ClZIP1) from Turmeric (Curcuma longa L.) and Expression Analysis in Presence of a Zn-Solubilizing Bacteria</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2022-09-01</date><risdate>2022</risdate><volume>40</volume><issue>3</issue><spage>500</spage><epage>515</epage><pages>500-515</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Zinc (Zn) deficiency is widespread in plants and molecular mechanism of uptake and transport within organelles is unclear. A novel Zn transporter gene was identified and characterized from turmeric (
Curcuma longa
L.) and expression analyzed in presence of zinc oxide (ZnO) and
Bacillus safensis
, a Zn-solubilizing bacteria (ZSB). Initially, eleven Zn transporters were mined from rhizome-specific transcriptome of turmeric and the one with highest transcript abundance designated as
ClZIP1
was cloned and sequenced to give 1268-bp gene encoding 366 amino acids (ORF-1101 bp). In silico analysis and deduced protein designated ClZIP1 to Zn/Fe-regulated transporter (ZRT/IRT)-related protein (ZIP) family with 70.0% identity to Zn transporters from
Musa acuminata
. ClZIP1 possessed eight transmembrane (TM) domains with a variable region between TM-3 and TM-4 and conserved histidine-rich ZIP signature motif. A single 10-bp Zn deficiency-responsive element (ZDRE) was present in the promoter.
ClZIP1
expression evaluated in in vitro plantlets in presence of Zn applied as ZnO (10–100 ppm), with/without ZSB indicated that it was higher in basal portion than leaf. This is the first report on ZIP gene from turmeric, which showed higher expression in the absence of ZSB and vice versa with maximum downregulation at 100 ppm Zn (88%).</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11105-021-01317-3</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-4020-4599</orcidid></addata></record> |
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| ispartof | Plant molecular biology reporter, 2022-09, Vol.40 (3), p.500-515 |
| issn | 0735-9640 1572-9818 |
| language | eng |
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| source | Springer Nature |
| subjects | Amino acids Aquatic plants Bacteria Bioinformatics Biomedical and Life Sciences Curcuma longa Histidine Life Sciences Metabolomics Nutrient deficiency Open reading frames Organelles Original Article Plant Breeding/Biotechnology Plant Sciences Plantlets Proteins Proteomics Regulatory sequences Rhizomes Transcriptomes Variable region Zinc oxide Zinc oxides |
| title | A Novel Zn Transporter Gene (ClZIP1) from Turmeric (Curcuma longa L.) and Expression Analysis in Presence of a Zn-Solubilizing Bacteria |
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