CRISPR/Cas12a-based photoelectrochemical sensing of microRNA on reduced graphene oxide-anchored Bi2WO6 coupling with catalytic hairpin assembly

• Published in: Sensors and actuators. B, Chemical Chemical, 2022-10, Vol.369, p.132307, Article 132307
• Main Authors: Gong, Hexiang, Hu, Xuehan, Zeng, Ruijin, Li, Yuxuan, Xu, Jianhui, Li, Meijin, Tang, Dianping
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Ascorbic acid; Assembly; Beads; Bi2WO6-based carbon materials; Biosensors; Bismuth compounds; Catalytic hairpin assembly; Circuits; Coupling; CRISPR; CRISPR/Cas12a-amplified system; Graphene; MicroRNAs; Nuclease; Photoelectric effect; Photoelectric emission; Photoelectrochemical analysis; Reduced graphene oxide; Ribonucleic acid; RNA; Selectivity; Tungstates
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2022.132307

An innovative and generic CRISPR/Cas12a-driven photoelectrochemical (PEC) biosensing platform was developed for screening of microRNA-21 (miR-21) by coupling with target-triggered catalytic hairpin assembly (CHA) and reduced graphene oxide-anchored Bi2WO6 (rGO-BWO) as the photoactive material. CHA isothermal amplification involved two programmable hairpin DNA modules and miR-21 as an activator. In the presence of miR-21, the products of target-triggered CHA circuit were inserted into the Cas12a-crRNA duplex to initial trans-cleavage capacity of CRISPR/Cas12a nuclease, accompanying the digestion on alkaline phosphatase (ALP)-labeled single-stranded DNA (ssDNA)-encoded magnetic bead (MB) through the activated CRISPR system. The ALPs were detached from magnetic beads and promoted the generation of ascorbic acid (AA), which increased the photocurrent of rGO-BWO-modified electrode. The value of photocurrent was positively proportional to the level of AA, which was also linearly correlated with target concentrations. Under optimum conditions, the CRISPR-based PEC sensing system displayed satisfying photocurrent responses toward miR-21 within the range from 1.0 fM to 1.0 nM with a limit of detection of 0.47 fM. In addition, the biosensor exhibited acceptable stability and excellent selectivity. Impressively, CHA-mediated CRISPR-based PEC biosensing platform provides a universal and sensitive method for clinical cancer diagnostics and biomolecular research. •An enhanced photoelectrochemical biosensor was designed for detection of microRNA.•Reduced graphene oxide-anchored Bi2WO6 used as the photoactive materials.•Bi2WO6-based carbon materials to enhance photoelectric conversion efficiency.•Catalytic hairpin assembly utilized for target recycling.•CRSIPR/Cas12 reaction system employed for in-situ amplified photocurrent.