Determination of Perfluororganic Compounds in Whole Blood and Tissues of Laboratory Animals by Gas-Liquid Chromatography–Mass Spectrometry

• Published in: Journal of analytical chemistry (New York, N.Y.) N.Y.), 2022-09, Vol.77 (9), p.1197-1202
• Main Authors: Zhurkovich, I. K., Utsal’, V. A., Ostrovidova, E. V., Barinov, V. A., Lugovkina, N. V., Bonitenko, E. Yu, Belyakova, N. A., Barinov, V. V.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Animals; Biomedical materials; Blood; Chemistry; Chemistry and Materials Science; Heptanes; In vivo methods and tests; Laboratory animals; Liquid chromatography; Liquid-liquid extraction; Mass spectrometry; Measurement; Methods; Organofluorine compounds; Perfluoro compounds; Perfluorodecalin; Physiological aspects; Spectroscopy
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934822070140

A procedure for the determination of a group of perfluororganic compounds, promising for medical use, in whole blood and tissues of laboratory animals by gas-liquid chromatography–mass spectrometry was developed in in vitro experiments. The sample preparation procedure based on the liquid–liquid extraction of the target compounds from the biomaterial with heptane containing perfluorobenzene as an internal standard was optimized. The validation of the procedure and its testing in in vivo experiments was carried out on an example of perfluorodecalin. The time of the presence of the xenobiotic in the bloodstream and in various organs of small laboratory animals during the endotracheal route of administration was estimated.