Phage Antibodies as Bioreceptors for Ampicillin Detection

Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibo...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and microbiology 2022-10, Vol.58 (5), p.646-651
Main Authors: Guliy, O. I., Alsowaidi, A. K. M., Fomin, A. S., Gabalov, K. P., Staroverov, S. A., Karavaeva, O. A.
Format: Article
Language:English
Subjects:
Ampicillin
Antibiotics
Antibodies
Biochemistry
Biomedical and Life Sciences
Immunoassay
Kanamycin
Life Sciences
Medical Microbiology
Microbiology
Penicillin
Phages
Phenylalanine
Sensory systems
Solid phases
Tryptophan
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683822050088