Photocaged Small Interfering RNA

Photoregulated systems are interesting and prospective instruments to affect gene expression. An approach to the synthesis of photoregulated siRNA has been proposed for RNA interference activated by light irradiation. For temporary protection, a bulky group has been introduced in siRNA through the p...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2022-10, Vol.48 (5), p.1036-1042
Main Authors: Akhmetova, E. A., Kim, D. V., Dome, A. S., Meschaninova, M. I., Novopashina, D. S.
Format: Article
Language:English
Subjects:
Activated carbon
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cholesterol
Fluorescence
Gene expression
Hydroxyl groups
Life Sciences
Ligands
Light irradiation
Organic Chemistry
Ribonucleic acid
RNA
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Summary:Photoregulated systems are interesting and prospective instruments to affect gene expression. An approach to the synthesis of photoregulated siRNA has been proposed for RNA interference activated by light irradiation. For temporary protection, a bulky group has been introduced in siRNA through the photocleavable linker. We have used amino-modified cholesterol and trileucine as bulky ligands, which have been attached to siRNA by the interaction with the hydroxyl group activated with disuccinimidyl carbonate. The efficient UV-induced removal of the ligands introduced through the photocleavable linker has been demonstrated. Using the proposed approach, siRNA molecules bearing cholesterol residues at the 5'-terminus of sense- and/or antisense strands have been synthesized. They were targeted to mRNA of EGFP (enhanced green fluorescent protein). The photoinduced inhibition of the EGFP gene expression in the EGFP-expressing HEK293FT cells has been shown to be possible.
ISSN:1068-1620
1608-330X
DOI:10.1134/S106816202205003X