Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish

In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture, especially in fish with relatively long generations. Nevertheless, functional sperm production from in vitro-cultured spermatogonia remains a ch...

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Bibliographic Details
Published in:Dōngwùxué yánjiū 2022-07, Vol.43 (4), p.537-551
Main Authors: Zhang, Hong, Zhang, Wan-Wan, Mo, Cheng-Yu, Dong, Meng-Dan, Jia, Kun-Tong, Liu, Wei, Yi, Mei-Sheng
Format: Article
Language:English
Subjects:
Aquaculture
Artificial insemination
Biological fertilization
Breeding
Cell culture
Differentiation
Fish
Fish culture
Fish reproduction
Gametes
Gametocytes
Growth factors
In vitro fertilization
Kinases
Larvae
Males
Marine fish
Marine fishes
Melatonin
Oocytes
Polyculture (aquaculture)
Proliferation
Reproduction (biology)
Signal transduction
Sperm
Spermatogenesis
Spermatogonia
Spermatozoa
Stem cells
Testes
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Summary:In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture, especially in fish with relatively long generations. Nevertheless, functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture fish. In this study, we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers (Bostrychus sinensis), which are prone to ovotesticular or sterile testicular development, and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional (3D) culture system. Artificial insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal larvae. Furthermore, melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway, and thus increased the efficiency in functional sperm production. The 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.
ISSN:0254-5853
DOI:10.24272/j.issn.2095-8137.2022.058