Occurrence and detection of Xanthomonas vasicola pv. vasculorum in naturally contaminated maize seeds in Brazil

Bacterial leaf streak of maize, caused by Xanthomonas vasicola pv. vasculorum , is an emerging disease in the USA and in South American countries. However, until recently its presence in maize seeds had not been detected in Brazil. The objective of this work was to detect X. vasicola pv. vasculorum...

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Published in:Tropical plant pathology 2022-10, Vol.47 (5), p.698-702
Main Authors: Tebaldi, Nilvanira Donizete, Pereira, Júlia Nunes Paniago, Sagata, Erika, Mota, Lara Caroline Borges Moreira, da Hora Junior, Braz Tavares, Piazzoli, Denis, Klosowski, Ana Cláudia
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Corn
Culture media
Cycloheximide
Dilution
Leaf streak
Leaves
Life Sciences
Pathogenicity
Pathogens
Plant bacterial diseases
Plant Pathology
Plant tissues
Seeds
Selective media
Short Communication
Signs and symptoms
Xanthomonas
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Summary:Bacterial leaf streak of maize, caused by Xanthomonas vasicola pv. vasculorum , is an emerging disease in the USA and in South American countries. However, until recently its presence in maize seeds had not been detected in Brazil. The objective of this work was to detect X. vasicola pv. vasculorum in maize seeds using a semi-selective culture medium. A bacterial suspension of X. vasicola pv. vasculorum was ten-fold serially diluted and plated on 523, YDC, or semi-selective NSCAA and XTS media, without or with the addition of cycloheximide. Bacteria detection in seeds was performed using samples from three seed lots collected from maize plants showing bacterial leaf streak symptoms. Seed washings were prepared, ten-fold serially diluted, and plated on the same four culture media. Addition of cycloheximide to 523, YDC, NSCAA, and XTS media did not inhibit bacterial growth. X. vasicola pv. vasculorum was detected in one maize seed sample using 523 and XTS media, which was confirmed by biochemical, physiological, and molecular characterization (Xvv7-F/Xvv7-R and Xvv8-F/Xvv8-R primers), as well as by pathogenicity tests. Semi-selective XTS medium with cycloheximide addition is recommended for routine laboratory analyses to detect X. vasicola pv. vasculorum in maize seeds and also for isolating from other potentially infected plant tissue. This is the first report of X. vasicola pv. vasculorum detection in naturally contaminated maize seeds in Brazil.
ISSN:1983-2052
1982-5676
1983-2052
DOI:10.1007/s40858-022-00514-z