Detecting cow milk in sheep yoghurt by TaqMan real‐time PCR

The detection of adulteration in the production of milk and other dairy products is crucial for economic reasons and consumer protection. In this study, yoghurts were produced from mixed milk (cow and sheep) in different ratios, and the percentage of sheep and cow milk was determined using TaqMan re...

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Bibliographic Details
Published in:International journal of dairy technology 2022-11, Vol.75 (4), p.803-808
Main Authors: Biçer, Yusuf, Sönmez, Gonca
Format: Article
Language:English
Subjects:
Authentication
Consumer protection
Cow's milk
Dairy products
Deoxyribonucleic acid
DNA
Milk
Polymerase chain reaction
qPCR
Sensitivity analysis
Sheep
Sheep yoghurt
Sheep's milk
TaqMan
Yogurt
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Summary:The detection of adulteration in the production of milk and other dairy products is crucial for economic reasons and consumer protection. In this study, yoghurts were produced from mixed milk (cow and sheep) in different ratios, and the percentage of sheep and cow milk was determined using TaqMan real‐time polymerase chain reaction (PCR). 1% of cow milk was determined in the yoghurts and 0.01 ng of cow and sheep DNA was determined in sensitivity analyses. Therefore, TaqMan real‐time PCR can be considered as a sensitive and reliable method for detecting cow DNA in yoghurt produced from mixed milk. Authentication of yoghurt produced with mixed cow and sheep milk by TaqMan qPCR.
ISSN:1364-727X
1471-0307
DOI:10.1111/1471-0307.12892