Insights into Profiling of 24-Epibrassinolide Treatment Alleviating the Loss of Glucosinolates in Harvested Broccoli

Postharvest yellowing is accompanied by the rapid nutrition deterioration of broccoli. Here, we investigated the effect of 24-epibrassinolide (EBR) treatment on the change of glucosinolates content during the yellowing of broccoli. EBR treatment maintained the content of glucosinolates, as indicated...

Full description

Saved in:
Bibliographic Details
Published in:Food and bioprocess technology 2022-12, Vol.15 (12), p.2697-2711
Main Authors: Yang, Qingxi, Luo, Manli, Zhou, Qian, Zhou, Xin, Zhao, Yingbo, Chen, Jianye, Ji, Shujuan
Format: Article
Language:English
Subjects:
Agriculture
Amino acids
Biosynthesis
Biotechnology
Brassinosteroids
Broccoli
Catalysis
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Food Science
Fruits
Glucosinolates
Kinases
Laboratories
Original Research
Polyethylene
Signal transduction
Transcription factors
Vegetables
Yellowing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Postharvest yellowing is accompanied by the rapid nutrition deterioration of broccoli. Here, we investigated the effect of 24-epibrassinolide (EBR) treatment on the change of glucosinolates content during the yellowing of broccoli. EBR treatment maintained the content of glucosinolates, as indicated by higher levels of glucoraphanin, glucobrassicin, and neoglucobrassicin. Meanwhile, the transcript levels of BoCYP83A1 and BoCYP83B1 as well as BoMYB28 , BoMYB34 , and BoMYB122 involved in the glucosinolates biosynthesis pathway were upregulated in the treatment sample. Furthermore, the expressions of BoBRI1 gene and crucial transcription factors BoBZR1 and BoBES1 that participated in the brassinosteroids signal transduction pathway were activated under EBR treatment, which indirectly alleviated the decrease of glucosinolates content. Orthogonal partial least square model and pathway analysis further demonstrated that the improvement of glucosinolate biosynthetic ability and the enhancement of brassinosteroids signal transduction might be the mechanism responsible for the conservation of glucosinolates by EBR treatment.
ISSN:1935-5130
1935-5149
DOI:10.1007/s11947-022-02909-x