Identification of insecticidal molecule aucubin from Metarhizium anisopliae ethyl acetate crude extract against disease mosquito vector

Identification of insecticidal compounds from the entomopathogenic fungus Metarhizium anisopliae crude extract was performed by TLC and column fractionation. From the fractionation four fractions were separated and tested for larvicidal, pupicidal and adulticidal potential against Aedes aegypti , An...

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Bibliographic Details
Published in:International journal of tropical insect science 2022, Vol.42 (5), p.3303-3318
Main Authors: Vivekanandhan, P., Swathy, K., Shivakumar, M. S.
Format: Article
Language:English
Subjects:
Acetic acid
Aedes aegypti
Anopheles stephensi
Aquatic insects
Biomedical and Life Sciences
Culex quinquefasciatus
Entomology
Entomopathogenic fungi
Ethyl acetate
Fractionation
Fungi
Infrared spectroscopy
Insecticides
Larvae
Life Sciences
Liquid chromatography
Metarhizium anisopliae
Microorganisms
Mosquitoes
NMR
Nuclear magnetic resonance
Original Research Article
Pesticides
Vectors
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Summary:Identification of insecticidal compounds from the entomopathogenic fungus Metarhizium anisopliae crude extract was performed by TLC and column fractionation. From the fractionation four fractions were separated and tested for larvicidal, pupicidal and adulticidal potential against Aedes aegypti , Anopheles stephensi and Culex quinquefasciatus mosquitoes . Among the four fractions the fraction-3 was most effective against larvae with LC 50 values, 1.238, 1.300 and 2.899 for pupae, 1.440, 1.657, 2.593 and adult, 1.988, 2.037 and 4.086 µg/ml against Ae. aegypti , An. stephensi and Cx. quinquefasciatus at 24 h post treatment respectively. The effective fraction-3 were charectrized through FT-IR, UV–Vis spectroscopy, 13 C-NMR and 1 H-NMR, HPLC, and LC–MS analysis allowed to identifying the effective compound in fraction-3. Overall, the results confirmed the use of the fungus derived molecules as an active green pesticide against mosquito vectors of public importance and led to the identification of highly effective mosquitocidal products. In particular the toxic activity of aucubin far encompassed that of the large majority of earlier studied microbial insecticides, making this molecule an ideal candidate for the development of insecticides in the “real world”.
ISSN:1742-7592
1742-7584
1742-7592
DOI:10.1007/s42690-022-00828-0