Application of Nucleotide-Based Kinetic Modeling Approaches to Predict Antibiotic Resistance Gene Degradation during UV- and Chlorine-Based Wastewater Disinfection Processes: From Bench- to Full-Scale

• Published in: Environmental science & technology 2022-11, Vol.56 (21), p.15141-15155
• Main Authors: He, Huan, Choi, Yegyun, Wu, Sean J., Fang, Xuzhi, Anderson, Annika K., Liou, Sin-Yi, Roberts, Marilyn C., Lee, Yunho, Dodd, Michael C.
• Format: Article
• Language: English
• Subjects: Ammonia; Antibiotic resistance; Antibiotics; Buffers; Chlorine; Deactivation; Degradation; Deoxyribonucleic acid; Disinfection; DNA; Drug resistance; E coli; Inactivation; Intracellular; Kinetics; Klebsiella; Monochloramine; Nucleotides; Pseudomonas aeruginosa; Rate constants; rRNA 16S; Segments; Strains (organisms); Treatment and Resource Recovery; Ultraviolet radiation; Wastewater treatment
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.2c00567

This study investigated antibiotic resistance gene (ARG) degradation kinetics in wastewaters during bench- and full-scale treatment with UV light and chlorinewith the latter maintained as free available chlorine (FAC) in low-ammonia wastewater and converted into monochloramine (NH2Cl) in high-ammonia wastewater. Twenty-three 142–1509 bp segments (i.e., amplicons) of seven ARGs (blt, mecA, vanA, tet(A), ampC, bla NDM, bla KPC) and the 16S rRNA gene from antibiotic resistant bacteria (ARB) strains Bacillus subtilis, Staphylococcus aureus, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae were monitored as disinfection targets by qPCR. Rate constants for ARG and 16S rRNA gene amplicon degradation by UV, FAC, and NH2Cl were measured in phosphate buffer and used to expand and validate several recently developed approaches to predict DNA segment degradation rate constants based solely on their nucleotide contents, which were then applied to model ARG degradation during bench-scale treatment in buffer and wastewater matrixes. Kinetics of extracellular and intracellular ARG degradation by UV and FAC were well predicted up to ∼1–2-log10 elimination, although with decreasing accuracy at higher levels for intracellular genes, while NH2Cl yielded minimal degradation under all conditions (agreeing with predictions). ARB inactivation kinetics varied substantially across strains, with intracellular gene degradation lagging cell inactivation in each case. ARG degradation levels observed during full-scale disinfection at two wastewater treatment facilities were consistent with bench-scale measurements and predictions, where UV provided ∼1-log10 ARG degradation, and chlorination of high-ammonia wastewater (dominated by NH2Cl) yielded minimal ARG degradation.