Effect of chitosan and hydroxyapatite nanocomposite on dentin erosion: An in-vitro study

Aim: To evaluate the dentinal tubule occlusion and collagen stabilization potential of nanohydroxyapatite (nHAp) and nanochitosan (nCH) combination paste on eroded dentin surface. Materials and Methods: In this in-vitro study, nHAp was prepared using the microwave-accelerated wet chemical synthesis...

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Bibliographic Details
Published in:Journal of international oral health 2022-09, Vol.14 (5), p.509-517
Main Authors: Gurucharan, Ishwarya, Derick Isaac, D, Madhubala, M, Vijay Amirtharaj, L, Mahalaxmi, Sekar, Jayasree, R, Sampath Kumar, T
Format: Article
Language:English
Subjects:
Chemical properties
Chitin
Chitosan
Citric acid
Collagen
Composite materials
Dental materials
Dental matrices
Dentin
Hydroxyapatite
Infrared spectroscopy
Light scattering
Mechanical properties
Molars
Nanocomposites
Nanoparticles
Occlusion
Physiological aspects
Scanning electron microscopy
Spectrum analysis
Statistical analysis
Testing
Tubules
X-ray spectroscopy
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Summary:Aim: To evaluate the dentinal tubule occlusion and collagen stabilization potential of nanohydroxyapatite (nHAp) and nanochitosan (nCH) combination paste on eroded dentin surface. Materials and Methods: In this in-vitro study, nHAp was prepared using the microwave-accelerated wet chemical synthesis method and nCH was made by the ionic gelation technique. The particles were characterized separately under dynamic light scattering and made into a paste by mixing them at a ratio of 1:1, which was further analyzed using Fourier-transform infrared spectroscopy (FTIR). Dentin slabs were prepared from 32 extracted human molars and subjected to erosion by exposing to 3% citric acid for 5 min. They were divided into four groups by convenience sampling method (n = 15): group I-control (no treatment); group II-nHAp; group III-nCH; group IV-nHA-nCH paste. All dentin samples were treated according to their respective groups by the active application of pastes using microbrushes for 1 min everyday for 14 days. Later, the samples were subjected to FTIR and scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDX) analysis. Statistical analysis was done using one-way analysis of variance (P < 0.05). Results: SEM-EDX revealed complete occlusion of dentinal tubules in the nHA-nCH group with HAp deposits on the surface. The Ca/P ratio of various groups was significantly different from each other (control group-1.05, nHAp-1.64, nCH-1.14, and nHA-nCH-1.71) (P < 0.05). The FTIR spectra marked the presence of amide I peak in nCH and nHA-nCH groups, indicating collagen stabilization. Conclusion: The nHA-nCH paste shows a potential for tubular occlusion and stabilizes both the inorganic and organic components of eroded dentin, respectively.
ISSN:0976-7428
0976-1799
DOI:10.4103/jioh.jioh_50_22