Exploration of the Beta-Actin DNA Integrity Index as Early Genetic Marker of Presence of Breast Cancer

Background: Circulating cell-free DNA (cfDNA) and its integrity index can be a fast and non-invasive (liquid biopsy) biomarker, this provides significant additional data for diagnosis, prognosis and therapy stratification in cancer patient. Methods: The circulating tumor DNA (ctDNA) concentration an...

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Bibliographic Details
Published in:Electronic journal of general medicine 2020-01, Vol.17 (2), p.em188
Main Authors: Habeeb, Wafaa Hussien, Suleiman, Ahmed AbdulJabbar, Al-Hitawee, Haitham Tafash
Format: Article
Language:English
Subjects:
Breast cancer
Colorectal cancer
Plasma
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Summary:Background: Circulating cell-free DNA (cfDNA) and its integrity index can be a fast and non-invasive (liquid biopsy) biomarker, this provides significant additional data for diagnosis, prognosis and therapy stratification in cancer patient. Methods: The circulating tumor DNA (ctDNA) concentration and integrity was investigated in the plasma from patients with breast cancer by a quantitative polymerase chain reaction (qPCR) and their diagnostic value for breast cancer etiology was evaluated Plasma samples were collected from 55 patients 40 patients with breast cancer, 5 patients each with other type of cancer (ovarian cancer, colon cancer, stomach cancer) and 20 healthy controls. Real-time PCR of β-actin gene were investigated using two primer sets (400 and 100bp) to amplify different DNA fragment lengths. The DNA integrity index was calculated as the ratio of q-PCR results of β-actin 400bp/100. Results: In all cancer patients the DNA concentrations were significantly higher p
ISSN:2516-3507
2516-3507
DOI:10.29333/ejgm/7618