A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetra...

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Bibliographic Details
Published in:Processes 2022-11, Vol.10 (11), p.2186
Main Authors: Wang, Wei-Yang, Wu, Shi-Hua, Xie, Yuan-Han, Zhong, Miao, Wei, Man-Li, Li, Ze-Yang, Long, Xiu-Feng, Niu, Fu-Xing
Format: Article
Language:English
Subjects:
Acids
Biofuels
Carbon
Cell growth
Chemical industry
Climate change
Emissions
Engineering
Ethanol
Evolution & development
Fermentation
Ferric nitrate
Fungi
Genomes
High-throughput screening
Industrial development
Iron
Medical research
Metabolism
Metabolites
Microorganisms
Mutagenesis
Mutants
Mutation
Nitrates
Pyruvic acid
Random mutagenesis
Saccharomyces cerevisiae
Screening
Sucrose
Yeast
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Summary:Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr10112186