Detection and identification of ‘Candidatus Phytoplasma palmae’ strains in Hortensia similis and Pritchardia pacifica in Cuba

Lethal yellowing is the most important disease affecting coconut production worldwide and in America it is associated with the presence of different strains or ribosomal 16S subgroups of the ‘ Candidatus Phytoplasma palmae’. A symptomatic ornamental palm plant species ( Pritchardia pacifica ) and in...

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Bibliographic Details
Published in:European journal of plant pathology 2022-12, Vol.164 (4), p.593-600
Main Authors: Paredes-Tomás, Camilo, Luis-Pantoja, Maritza, Rodríguez-Tapia, Jorge Luis, Salin, Carlos Oropeza, Narváez, María, Myrie, Wayne, Pacini, Francesco, Bertaccini, Assunta
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Candidatus Phytoplasma palmae
Cloning
Ecology
Enzymes
Insects
Life Sciences
Maximum likelihood method
Mixed infection
Ornamental plants
Phylogenetics
Phytoplasma
Plant bacterial diseases
Plant Pathology
Plant Sciences
Plant species
Polymerase chain reaction
Pritchardia
Ribosomal DNA
rRNA 16S
Subgroups
Yellowing
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Summary:Lethal yellowing is the most important disease affecting coconut production worldwide and in America it is associated with the presence of different strains or ribosomal 16S subgroups of the ‘ Candidatus Phytoplasma palmae’. A symptomatic ornamental palm plant species ( Pritchardia pacifica ) and individuals of the insect  Hortensia similis were sampled in Cuba from selected areas to verify their possible role in the maintenance and dissemination of this phytoplasma. Both, plant and insect were positive for ‘ Ca . P. palmae’ presence as cross verified by RFLP analyses on the 16S rRNA and groEl genes specific amplicons. The cloned sequences of phytoplasmas detected in H. similis are enclosed in the 16SrIV-A subgroup, while those from P. pacifica had different profiles with Alu I, Hha I and Sau3 A enzymes. Further RFLP analyses on 16S rDNA amplicons confirmed the presence of 16SrIV-A phytoplasmas also in P. pacifica suggesting a possible presence of a mixed infection of diverse phytoplasma strains/lineages. This is the first record of the occurrence of a ‘ Ca . P. palmae’ strains in P. pacifica in Cuba and of H. similis as potential insect vector of this phytoplasma.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-022-02565-z