A tri-response colorimetric-fluorescent probe for pH and lysosomal imaging

• Published in: Sensors and actuators. B, Chemical Chemical, 2022-11, Vol.370, p.132425, Article 132425
• Main Authors: Mei, Huihui, Gu, Xin, Wang, Minghui, Chen, Jiajia, Yang, Xindi, Liu, Xuejun, Xu, Kuoxi
• Format: Article
• Language: English
• Subjects: Biological activity; Cells (biology); Colorimetric-fluorescent probe; Colorimetry; Fluorescent indicators; Imaging; Lysosomal detection; PH detection; Tri-response probe
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2022.132425

Intracellular pH has been associated with multiple biological processes. However, fluorescent probes for pH are limited. Herein, a new pH fluorescent probe was developed based on reversible structural changes. This probe provided three reversible pH response modes within 2.00–12.00. Under the acidic conditions, there is no-fluorescence, under the neutral conditions, the emission is at 650 nm, under the alkaline conditions, the emissions at 650 nm decreases and at 460 nm appears and increases simultaneously. The probe solution presented distinct colors response to the pH values from 2.00 to 12.00. The fluorescent response from pH 2.00–12.00 makes the probe suitable for the monitoring pH in living cells. Moreover, the imaging results confirm that probe S can be applied in fluorescence imaging in living cells. •A new pH fluorescent probe was developed based on reversible structural changes.•The probe provided three reversible pH response modes accompanied by different color changes.•The probe can be applied to live cell pH monitoring and lysosome imaging.