Engineering a thermostable biosensor based on biomimetic mineralization HRP@Fe-MOF for Alzheimer’s disease

The development of disease detection by biosensors represents one of the key components of medical science. However, millions of people are still misdiagnosed each year due to the poor efficacy and thermal instability of biosensors. Using horseradish peroxidase (HRP) as a paradigm, we offer a ration...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2022-12, Vol.414 (29-30), p.8331-8339
Main Authors: Miao, Yang-Bao, Zhong, Qilong, Ren, Hong-Xia
Format: Article
Language:English
Subjects:
Alzheimer's disease
Analysis
Analytical Chemistry
Aptamers
Biochemistry
Biomimetics
Biosensors
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Design optimization
Diagnosis
Disease detection
Enzymes
Food Science
Heat treatment
Heat treatments
High temperature
Horseradish peroxidase
Hydrogen peroxide
Identification and classification
Laboratory Medicine
Medical science
Methods
Mineralization
Monitoring/Environmental Analysis
Neurodegenerative diseases
Organometallic compounds
Peroxidase
Research Paper
Thermal instability
Thermal properties
Thermal stability
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Summary:The development of disease detection by biosensors represents one of the key components of medical science. However, millions of people are still misdiagnosed each year due to the poor efficacy and thermal instability of biosensors. Using horseradish peroxidase (HRP) as a paradigm, we offer a rational design strategy to optimize the thermostability and activity of biosensors by biomimetic mineralization. To overcome the weak thermostability of the biosensor, the mineralization of Fe-MOF forms an armor on HRP that protects against high temperature. Additionally, the biomimetic mineralization HRP@Fe-MOF can double-catalyze the TMB/H 2 O 2 chromogenic system for color development. The biosensor can also be recycled through simple heat treatment due to the thermally stable aptamer and biomimetic mineralization HRP@Fe-MOF. The optical biosensor based on this sensitive spectral transformation was successfully developed for the measurement of AβO with an outstanding linear range (0.0001–10 nM) and a low limit of detection (LOD) of 0.03 pM. This promising platform will open up new avenues for the detection of AβO in the early diagnosis of Alzheimer’s disease (AD).
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-022-04367-y