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Automated sample preparation and fast GC–MS determination of fatty acids in blood samples and dietary supplements
The present research is focused on the optimization of an automatized sample preparation and fast gas chromatography–mass spectrometry (GC-MS) method for the analysis of fatty acid methyl esters (FAMEs) in blood samples and dietary supplements, with the primary objective being a significant reductio...
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| Published in: | Analytical and bioanalytical chemistry 2022-12, Vol.414 (29-30), p.8423-8435 |
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| cites | cdi_FETCH-LOGICAL-c349t-927f9f5430b3267c68517be5fdad630a7f09f87a4b0a7889d1ab6870de8a6ebe3 |
| container_end_page | 8435 |
| container_issue | 29-30 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Ferracane, Antonio Aloisi, Ivan Galletta, Micaela Zoccali, Mariosimone Tranchida, Peter Q. Micalizzi, Giuseppe Mondello, Luigi |
| description | The present research is focused on the optimization of an automatized sample preparation and fast gas chromatography–mass spectrometry (GC-MS) method for the analysis of fatty acid methyl esters (FAMEs) in blood samples and dietary supplements, with the primary objective being a significant reduction of the analysis time and, hence, an enhanced sample throughput. The mass spectrometer was operated in the scan/selected ion monitoring (SIM) acquisition method, thus enabling the obtainment of qualitative and (highly sensitive) quantitative data. The separation of FAMEs was obtained in about 11 min by using a micro-bore column of dimensions 15 m × 0.10 mm ID × 0.10 µm d
f
with a polyethylene glycol stationary phase. The novelty of the research involves reducing analysis time by using the novel fast GC–MS method with increased identification reliability and sensitivity in a single chromatographic run. With regard to the figures of merit, linearity, accuracy, and limits of detection (LoD) and quantification (LoQ) were determined. Specifically, regression coefficients were between 0.9901 and 0.9996; the LoDs ranged from 0.05 to 1.02 µg g
−1
for the blood analysis method, and from 0.05 to 0.26 mg g
−1
in the case of the dietary supplement approach. With respect to LoQs, the values were in the ranges of 0.15–3.39 µg g
−1
and 0.15–0.86 mg g
−1
for blood and dietary supplements analysis methods, respectively. Accuracy was evaluated by analyzing certified reference materials (human plasma, fish oil).
Graphical abstract |
| doi_str_mv | 10.1007/s00216-022-04379-8 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2742879227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A728466689</galeid><sourcerecordid>A728466689</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-927f9f5430b3267c68517be5fdad630a7f09f87a4b0a7889d1ab6870de8a6ebe3</originalsourceid><addsrcrecordid>eNp9kc1KxDAQx4soqKsv4CnguZqkbZIel8UvUDyo55A2kyWyTWqSPXjzHXxDn8S49QNBZA4zzPx_MyH_ojgi-IRgzE8jxpSwElNa4rribSm2ij3CiCgpa_D2d13T3WI_xkeMSSMI2yvifJ38oBJoFNUwrgCNAUYVVLLeIeU0MiomdLF4e3m9uUMaEoTBumnsTZ6m9IxUb3VE1qFu5f3XprjBtYWkwjOK6zH3BnApHhQ7Rq0iHH7mWfFwfna_uCyvby-uFvPrsq_qNpUt5aY1TV3hrqKM90w0hHfQGK00q7DiBrdGcFV3uRai1UR1THCsQSgGHVSz4njaOwb_tIaY5KNfB5dPSsprKnhLKf9RLdUKpHXGp6D6wcZezjkVNWNMtFl18ocqh4bB9t6Bsbn_C6AT0AcfYwAjx2CH_BOSYPnhmZw8k9kzufFMigxVExSz2C0h_Lz4H-odageanQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2742879227</pqid></control><display><type>article</type><title>Automated sample preparation and fast GC–MS determination of fatty acids in blood samples and dietary supplements</title><source>Springer Nature</source><creator>Ferracane, Antonio ; Aloisi, Ivan ; Galletta, Micaela ; Zoccali, Mariosimone ; Tranchida, Peter Q. ; Micalizzi, Giuseppe ; Mondello, Luigi</creator><creatorcontrib>Ferracane, Antonio ; Aloisi, Ivan ; Galletta, Micaela ; Zoccali, Mariosimone ; Tranchida, Peter Q. ; Micalizzi, Giuseppe ; Mondello, Luigi</creatorcontrib><description>The present research is focused on the optimization of an automatized sample preparation and fast gas chromatography–mass spectrometry (GC-MS) method for the analysis of fatty acid methyl esters (FAMEs) in blood samples and dietary supplements, with the primary objective being a significant reduction of the analysis time and, hence, an enhanced sample throughput. The mass spectrometer was operated in the scan/selected ion monitoring (SIM) acquisition method, thus enabling the obtainment of qualitative and (highly sensitive) quantitative data. The separation of FAMEs was obtained in about 11 min by using a micro-bore column of dimensions 15 m × 0.10 mm ID × 0.10 µm d
f
with a polyethylene glycol stationary phase. The novelty of the research involves reducing analysis time by using the novel fast GC–MS method with increased identification reliability and sensitivity in a single chromatographic run. With regard to the figures of merit, linearity, accuracy, and limits of detection (LoD) and quantification (LoQ) were determined. Specifically, regression coefficients were between 0.9901 and 0.9996; the LoDs ranged from 0.05 to 1.02 µg g
−1
for the blood analysis method, and from 0.05 to 0.26 mg g
−1
in the case of the dietary supplement approach. With respect to LoQs, the values were in the ranges of 0.15–3.39 µg g
−1
and 0.15–0.86 mg g
−1
for blood and dietary supplements analysis methods, respectively. Accuracy was evaluated by analyzing certified reference materials (human plasma, fish oil).
Graphical abstract</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-022-04379-8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Biochemistry ; Blood ; Blood plasma ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Composition ; Dietary supplements ; Esters ; Fatty acid methyl esters ; Fatty acids ; Fish oils ; Food Science ; Gas chromatography ; Ions ; Laboratory Medicine ; Mass spectrometry ; Mass spectroscopy ; Monitoring/Environmental Analysis ; Optimization ; Polyethylene glycol ; Qualitative analysis ; Regression coefficients ; Research Paper ; Sample preparation ; Stationary phase</subject><ispartof>Analytical and bioanalytical chemistry, 2022-12, Vol.414 (29-30), p.8423-8435</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>COPYRIGHT 2022 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-927f9f5430b3267c68517be5fdad630a7f09f87a4b0a7889d1ab6870de8a6ebe3</citedby><cites>FETCH-LOGICAL-c349t-927f9f5430b3267c68517be5fdad630a7f09f87a4b0a7889d1ab6870de8a6ebe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Ferracane, Antonio</creatorcontrib><creatorcontrib>Aloisi, Ivan</creatorcontrib><creatorcontrib>Galletta, Micaela</creatorcontrib><creatorcontrib>Zoccali, Mariosimone</creatorcontrib><creatorcontrib>Tranchida, Peter Q.</creatorcontrib><creatorcontrib>Micalizzi, Giuseppe</creatorcontrib><creatorcontrib>Mondello, Luigi</creatorcontrib><title>Automated sample preparation and fast GC–MS determination of fatty acids in blood samples and dietary supplements</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><description>The present research is focused on the optimization of an automatized sample preparation and fast gas chromatography–mass spectrometry (GC-MS) method for the analysis of fatty acid methyl esters (FAMEs) in blood samples and dietary supplements, with the primary objective being a significant reduction of the analysis time and, hence, an enhanced sample throughput. The mass spectrometer was operated in the scan/selected ion monitoring (SIM) acquisition method, thus enabling the obtainment of qualitative and (highly sensitive) quantitative data. The separation of FAMEs was obtained in about 11 min by using a micro-bore column of dimensions 15 m × 0.10 mm ID × 0.10 µm d
f
with a polyethylene glycol stationary phase. The novelty of the research involves reducing analysis time by using the novel fast GC–MS method with increased identification reliability and sensitivity in a single chromatographic run. With regard to the figures of merit, linearity, accuracy, and limits of detection (LoD) and quantification (LoQ) were determined. Specifically, regression coefficients were between 0.9901 and 0.9996; the LoDs ranged from 0.05 to 1.02 µg g
−1
for the blood analysis method, and from 0.05 to 0.26 mg g
−1
in the case of the dietary supplement approach. With respect to LoQs, the values were in the ranges of 0.15–3.39 µg g
−1
and 0.15–0.86 mg g
−1
for blood and dietary supplements analysis methods, respectively. Accuracy was evaluated by analyzing certified reference materials (human plasma, fish oil).
Graphical abstract</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Blood</subject><subject>Blood plasma</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composition</subject><subject>Dietary supplements</subject><subject>Esters</subject><subject>Fatty acid methyl esters</subject><subject>Fatty acids</subject><subject>Fish oils</subject><subject>Food Science</subject><subject>Gas chromatography</subject><subject>Ions</subject><subject>Laboratory Medicine</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Monitoring/Environmental Analysis</subject><subject>Optimization</subject><subject>Polyethylene glycol</subject><subject>Qualitative analysis</subject><subject>Regression coefficients</subject><subject>Research Paper</subject><subject>Sample 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sample preparation and fast GC–MS determination of fatty acids in blood samples and dietary supplements</title><author>Ferracane, Antonio ; Aloisi, Ivan ; Galletta, Micaela ; Zoccali, Mariosimone ; Tranchida, Peter Q. ; Micalizzi, Giuseppe ; Mondello, Luigi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-927f9f5430b3267c68517be5fdad630a7f09f87a4b0a7889d1ab6870de8a6ebe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Blood</topic><topic>Blood plasma</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composition</topic><topic>Dietary supplements</topic><topic>Esters</topic><topic>Fatty acid methyl esters</topic><topic>Fatty acids</topic><topic>Fish oils</topic><topic>Food 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supplements</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>414</volume><issue>29-30</issue><spage>8423</spage><epage>8435</epage><pages>8423-8435</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The present research is focused on the optimization of an automatized sample preparation and fast gas chromatography–mass spectrometry (GC-MS) method for the analysis of fatty acid methyl esters (FAMEs) in blood samples and dietary supplements, with the primary objective being a significant reduction of the analysis time and, hence, an enhanced sample throughput. The mass spectrometer was operated in the scan/selected ion monitoring (SIM) acquisition method, thus enabling the obtainment of qualitative and (highly sensitive) quantitative data. The separation of FAMEs was obtained in about 11 min by using a micro-bore column of dimensions 15 m × 0.10 mm ID × 0.10 µm d
f
with a polyethylene glycol stationary phase. The novelty of the research involves reducing analysis time by using the novel fast GC–MS method with increased identification reliability and sensitivity in a single chromatographic run. With regard to the figures of merit, linearity, accuracy, and limits of detection (LoD) and quantification (LoQ) were determined. Specifically, regression coefficients were between 0.9901 and 0.9996; the LoDs ranged from 0.05 to 1.02 µg g
−1
for the blood analysis method, and from 0.05 to 0.26 mg g
−1
in the case of the dietary supplement approach. With respect to LoQs, the values were in the ranges of 0.15–3.39 µg g
−1
and 0.15–0.86 mg g
−1
for blood and dietary supplements analysis methods, respectively. Accuracy was evaluated by analyzing certified reference materials (human plasma, fish oil).
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00216-022-04379-8</doi><tpages>13</tpages></addata></record> |
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| subjects | Analysis Analytical Chemistry Biochemistry Blood Blood plasma Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Composition Dietary supplements Esters Fatty acid methyl esters Fatty acids Fish oils Food Science Gas chromatography Ions Laboratory Medicine Mass spectrometry Mass spectroscopy Monitoring/Environmental Analysis Optimization Polyethylene glycol Qualitative analysis Regression coefficients Research Paper Sample preparation Stationary phase |
| title | Automated sample preparation and fast GC–MS determination of fatty acids in blood samples and dietary supplements |
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