Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

• Published in: Euphytica 2023, Vol.219 (1), p.2, Article 2
• Main Authors: Souza, Analu Cruz, Souza, Margarete Magalhães, Silva, Gonçalo Santos, Lavinscky, Manuella Palmeira, de Oliveira Souza, Viviane, de Oliveira Freitas, Jôsie Cloviane
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biotechnology; Cell culture; Chiasma frequency; Chromosomes; Disorientation; Fertility; Fibers; Fluorescein; Fluorescein diacetate; Genotypes; Germination; Hybrids; Interspecific hybridization; Life Sciences; Males; Meiosis; Ornamental plants; Passiflora; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Pollen; Reproduction (biology); Spindles
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-022-03110-z

Interspecific hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized . Passiflora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, artificial Passiflora hybrids were developed without many difficulties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Confirmation of interspecific hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander`s solution, fluorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n  = 9 with nine bivalent (II) chromosomal, and pairing in 90% of the cells in diakinesis. There was a significant difference ( p