Endomembranes, cytoskeleton, and cell walls: aspects of the ultrastructure of the vascular cambium of taproots of Aesculus hippocastanum L. (Hippocastanaceae)

• Published in: International journal of plant sciences 1997-03, Vol.158 (2), p.97-109
• Main Authors: Chaffey, Nigel, Barnett, John, Barlow, Peter
• Format: Article
• Language: English
• Subjects: aesculus; biochemistry; biochimie; bioquimica; Botany; Cambium; Cell membranes; Cell nucleus; cell structure; Cell walls; cells; Cellular biology; cellule; celulas; Coated vesicles; cytoplasmic organelles; Daughter cells; Development; estructura celular; Flowers & plants; Microtubules; organite cellulaire; organulos citoplasmicos; pared celular; paroi cellulaire; Plant cells; Plant growth; Plants; Polysaccharides; racine; raices; roots; structure cellulaire; ultraestructura; ultrastructure
• ISSN: 1058-5893; 1537-5315
• DOI: 10.1086/297419

The ultrastructure of ray and fusiform cells within the active vascular cambial zone of taproots of Aesculus hippocastanum L. is described. Both cell types are uninucleate and highly vacuolate and contain perinuclear and parietal populations of plastids and mitochondria, abundant rough endoplasmic reticulum, numerous dictyosomes and associated smooth vesicles, coated vesicles, numerous free ribosomes and polysomes, axially oriented microfilament bundles, and randomly oriented cortical microtubules, and they bear unlignified primary cell walls. Oleosomes, microbodies, and amyloplasts are more common within ray cells than in the fusiform cells and provide the main ultrastructural difference between the two. Generally, the ultrastructure of root cambial tissue is similar to the shoot cambium of this species and to the shoot cambia of other hardwood species. On the basis of ultrastructure, it has not been possible to identify true cambial initials in this tissue. Aspects of wall biosynthesis and chemistry were studied with light microscopy, conventional transmission electron microscopy, periodic acid-thiocarbohydrazide-silver proteinate staining (PATAg, Thiery reaction), and indirect immunolocalization, using the monoclonal antibodies JIM5 and JIM7 at the optical and ultrastructural levels. The presence of coated vesicles attached to a membranous structure is reported and discussed in relation to its identification as partially coated reticulum or trans-Golgi network. The results are discussed in relation to the endomembrane system, cell walls, and cytoskeleton of this tissue.