Dual ligand–capped gold nanoclusters for the smart detection of specific reactive oxygen species

  Quantitative detection of different types of reactive oxygen species (ROS) is vital for understanding the crucial roles of them in biological processes. However, few researches achieved the detection of multiple types of ROS with one probe until now. Given this, we designed and prepared fluorescen...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-01, Vol.190 (1), p.14, Article 14
Main Authors: Liu, Hengyu, Nan, Zhezhu, Zhu, Hongrui, Chen, Junjie, Ilovitsh, Tali, Wu, Daocheng, Wan, Mingxi, Feng, Yi
Format: Article
Language:English
Subjects:
Albumin
Analytical Chemistry
Ascorbic acid
Biological activity
Body fluids
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Crosslinking
Fluorescent indicators
Gold
Hydrogen Peroxide
Ligands
Lysozyme
Microengineering
Nanochemistry
Nanoclusters
Nanotechnology
Organic acids
Original Paper
Oxygen
Reactive Oxygen Species
Serum albumin
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Summary:  Quantitative detection of different types of reactive oxygen species (ROS) is vital for understanding the crucial roles of them in biological processes. However, few researches achieved the detection of multiple types of ROS with one probe until now. Given this, we designed and prepared fluorescent gold nanoclusters capped by dual ligand bovine serum albumin and lysozyme (BSA-LYS-AuNCs), which could detect 3 specific types of ROS based on its different fluorescent responses to H 2 O 2 , •OH and ClO − , respectively. The limit of detection (LOD) of H 2 O 2 , •OH, and ClO − was as low as 0.82 μM, 0.45 μM, and 0.62 μM. Moreover, as an important ROS type, ClO − was detected with high sensitivity and low LOD by BSA-LYS-AuNCs. It was also proved that the crosslinking of protein mainly contributed to the unique fluorescent characteristics of the probe exposing to ClO − . Furthermore, the fluorescent probe achieved the smart detection of hROS (including •OH and ClO − ) and wROS (the form of H 2 O 2 ) in the real sample, which could also been applied specifically to the detection of antioxidants, e.g. ascorbic acid. The gold nanoclusters developed have high potential for the smart detection of multiple ROS in the body fluid of organisms. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-022-05583-0