Are Plants Capable of Pheomelanin Synthesis? Gas Chromatography/Tandem Mass Spectrometry Characterization of Thermally Degraded Melanin Isolated from Echinacea purpurea

Echinacea is a widely used plant medicine, valued especially for its well-documented ability to stimulate the immune system. It has been suggested that melanin could be one of the bioactive factors responsible for the immunostimulatory properties of the plant. The biological functions of melanin pig...

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Bibliographic Details
Published in:Processes 2022-11, Vol.10 (11), p.2465
Main Authors: Kurkiewicz, Slawomir, Marek, Łukasz, Kurkiewicz, Małgorzata, Kurkiewicz, Adam, Dzierżęga-Lęcznar, Anna
Format: Article
Language:English
Subjects:
Biological activity
Chemical composition
Chromatography
Degradation products
Echinacea purpurea
Gas chromatography
Herbs
Hydrochloric acid
Immune system
Immunostimulation
Mass spectrometry
Mass spectroscopy
Medicinal plants
Melanin
Nitrogen
Oxidation
Pheomelanin
Pigments
Quadrupoles
Scientific imaging
Sulfur
Thermal degradation
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Summary:Echinacea is a widely used plant medicine, valued especially for its well-documented ability to stimulate the immune system. It has been suggested that melanin could be one of the bioactive factors responsible for the immunostimulatory properties of the plant. The biological functions of melanin pigments are closely related to their chemical composition and structural features. The aim of this study was to characterize the melanin from Echinacea purpurea based on the analysis of thermal degradation products of the well-purified pigment extracted from the dried herb. The melanin was pyrolyzed, and the resulting products were separated by gas chromatography and identified using a triple quadrupole mass spectrometer operating in full scan and multiple reaction monitoring modes. Three groups of marker products were detected in the melanin pyrolysate: polyphenol derivatives, nitrogen-containing heterocycles devoid of sulfur, and benzothiazines/benzothiazoles. This suggests that E. purpurea produces three structurally different melanin pigments: allomelanin, eumelanin, and pheomelanin, which in turn may affect the biological activity of the herb. Our results provide the first-ever evidence that plants are capable of synthesizing pheomelanin, which until now, has only been described for representatives of the animal and fungal kingdoms.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr10112465