Solvent optimization for efficient enzymatic monoacylglycerol production based on a glycerolysis reaction

This study was aimed at screening solvent systems of varying polarities to identify suitable solvents for efficient and practical enzymatic glycerolysis. Several pure solvents and solvent mixtures were screened in a batch reaction system consisting of glycerol, sunflower oil, and Novozym® 435 lipase...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Oil Chemists' Society 2005-08, Vol.82 (8), p.559-564
Main Authors: Damstrup, M.L, Jensen, T, Sparso, F.V, Kiil, S.Z, Jensen, A.D, Xu, X
Format: Article
Language:English
Subjects:
Alcohol
and tertiary alcohols
Biological and medical sciences
Candida antarctica lipase
Enzymes
Fat industries
food biotechnology
Food industries
food industry
Fundamental and applied biological sciences. Psychology
glycerol
glycerolysis
monoacylglycerols
Oils & fats
Optimization
organic solvents
Solvents
Sunflower oil
triacylglycerol lipase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was aimed at screening solvent systems of varying polarities to identify suitable solvents for efficient and practical enzymatic glycerolysis. Several pure solvents and solvent mixtures were screened in a batch reaction system consisting of glycerol, sunflower oil, and Novozym® 435 lipase. Out of 13 solvents tested, tert‐butanol and tert‐pentanol were the only pure solvents suitable for a fast glycerolysis reaction with an acceptably high formation of MAG. In these systems, MAG contents of 68–82% were achieved within a few hours. Mixtures of tert‐butanol/hexane, tert‐pentanol/hexane, and tert‐butanol/tert‐pentanol in varying ratios also gave high MAG contents (58–78%). The tertiary alcohols tert‐butanol and tert‐pentanol, or mixtures of one of them with hexane, seemed to be the best choice among the solvents tested with respect to reaction efficiency, practical industrial applications, and steric hydroxyl group hindrance, which suppresses the ester formation with FA.
ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-005-1109-y