Heat-killed Lactobacillus paracasei K71 isolated from Sakekasu (sake lees) enhances Th1 response, NK cell cytotoxicity and fecal IgA content in normal BALB/c mice

Lactobacillus paracasei K71 (K71) isolated from Japanese sake lees (Sakekasu) was previously shown to enhance IL-12 production in mouse spleen cell culture. In this study, we examined whether heat-killed K71 exerts immunostimulatory effects in normal BALB/c mice. Six-week dietary supplementation wit...

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Bibliographic Details
Published in:FOOD SCIENCE AND TECHNOLOGY RESEARCH 2022, Vol.28(4), pp.335-341
Main Authors: Hara, Takashi, Igarashi, Akira, Teradaira, Takuya, Joh, Toshio
Format: Article
Language:English
Subjects:
Cell culture
Cytotoxicity
Dietary supplements
fecal IgA
Heat
IFN-γ
Immune response
Immunoglobulin A
Immunoglobulin G
Immunostimulation
Innate immunity
Interleukin 12
Interleukin 4
Lactobacilli
Lactobacillus paracasei
Lactobacillus paracasei K71
Lymphocytes T
Natural killer cells
NK cells
Spleen
Th1 type immune responses
Toxicity
Tumorigenesis
γ-Interferon
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Summary:Lactobacillus paracasei K71 (K71) isolated from Japanese sake lees (Sakekasu) was previously shown to enhance IL-12 production in mouse spleen cell culture. In this study, we examined whether heat-killed K71 exerts immunostimulatory effects in normal BALB/c mice. Six-week dietary supplementation with 0.2% heat-killed K71 resulted in significantly higher levels of serum IgG2a and fecal IgA than control mice. Upon administration, heat-killed K71 increased IFN-γ production in Peyer's patch cells while simultaneously suppressing IL-4 production in spleen cells. Spleen cells from mice fed heat-killed K71 exhibited a significant increase in cytotoxicity against YAC-1 cells. Furthermore, splenic CD49b+NKp46+ NK cells from mice fed heat-killed K71 showed significantly enhanced IFN-γ production. These results suggest that oral intake of K71 may augment Th1 type immune function and innate immunity, thereby potentially preventing infections and/or tumorigenesis.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.FSTR-D-21-00290