P58IPK facilitates plant recovery from ER stress by enhancing protein synthesis

P58 IPK has been implicated in eukaryotic ER stress responses and viral pathogenesis, however, its biological functions and molecular mechanism in plants are unclear. Prolonged ER stress produced by tunicamycin (TM) increased P58 IPK mRNA and protein levels in Arabidopsis. Although the growth of 2  ...

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Published in:Plant biotechnology reports 2022-12, Vol.16 (6), p.665-681
Main Authors: Ko, Ki Seong, Yoo, Jae Yong, Kim, Kyung Hwa, Hwang, Bo Young, Vu, Bich Ngoc, Lee, Young Eun, Choi, Ha Na, Lee, Yoo Na, Yun, Jihee, Park, Ji Ye, Chung, Woo Sik, Hong, Jong Chan, Jeong, Myeong Seon, Jung, Hyun Suk, Jung, Su Kyoung, Park, Jeong Mee, Lee, Kyun Oh
Format: Article
Language:English
Subjects:
Agriculture
Arabidopsis
Biomedical and Life Sciences
Biotechnology
Cell Biology
Life Sciences
mRNA
Mutants
Myc protein
Original Article
Pathogenesis
Plant Biochemistry
Plant Sciences
Protein biosynthesis
Protein folding
Protein synthesis
Proteins
Recovery
Ribonucleic acid
Ribosomes
RNA
Tunicamycin
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Summary:P58 IPK has been implicated in eukaryotic ER stress responses and viral pathogenesis, however, its biological functions and molecular mechanism in plants are unclear. Prolonged ER stress produced by tunicamycin (TM) increased P58 IPK mRNA and protein levels in Arabidopsis. Although the growth of 2  ×  35S:P58 IPK -myc plants was less severely inhibited than that of Col-0 plants, TM inhibited the growth of p58 ipk -2 mutants more severely than that of Col-0 plants. Under prolonged ER stress conditions, the unfolded protein response (UPR)-related genes were expressed at a higher level in the p58 ipk -2 mutants than in Col-0 plants. Protein synthesis inhibition by TM in 2  ×  35S:P58 IPK -myc plants was lower than in Col-0 plants under prolonged ER stress conditions, however, not significantly different in p58 ipk -2 mutants. The GST-P58 IPK protein exhibited both chaperone and RNA-binding activities in a dose-dependent manner. P58 IPK has been shown to interact with ribosomes, allowing for enhanced protein production on the ER membrane. Following ER stress, 2  ×  35S:P58 IPK -myc plants recovered better than Col-0, but p58 ipk -2 mutants recovered less than Col-0. These findings reveal that P58 IPK can promote protein translation in association with ribosomes and contribute to stress recovery in Arabidopsis when induced during the last phase of ER stress.
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-022-00797-3