In-Vitro Antioxidant, Anti-Inflammatory and Cytotoxic effects of different Solvent Extraction Terminalia chebula, Terminalia billerica, Phyllanthus emblica

Objective: The phytochemical analysis of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica fruit extract as well as to evaluate the potential synergistic activity of screened plant extracts for anti-oxidant, anti-inflammatory and anti-cancer activity on MCF-7 breast cancer cell line. Met...

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Published in:Research journal of pharmacy and technology 2022-07, Vol.15 (7), p.2940-2944
Main Authors: K. Patel, Sanakousar, K Shutter, Arun, Patil, Ria, Desangi, Archana, Malali, Vishalakshi, Patil, Jyoti, Patil, Sumangala, K. Das, Kusal, P. Parvatikar, Prachi
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Breast cancer
Cell growth
Cytotoxicity
Ethanol
Flavonoids
Free radicals
Herbal medicine
Hydrogen peroxide
Morphology
Nonsteroidal anti-inflammatory drugs
Phenols
Phytochemicals
Proteins
Solvent extraction processes
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Summary:Objective: The phytochemical analysis of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica fruit extract as well as to evaluate the potential synergistic activity of screened plant extracts for anti-oxidant, anti-inflammatory and anti-cancer activity on MCF-7 breast cancer cell line. Methodology: In present study phytochemical constituent from selected plant material was extracted in different solvents. Antioxidant activity was evaluated by DPPH and FRAP assay against standard antioxidant. MTT assay was performed to find out cytotoxic and anti-inflammatory effect on MCF- breast cancer cell line. Result: Phytochemical analysis of the performed which showed that alkaloids, phenols, flavonoids and saponins. In context of antioxidant assays almost selected extracts showed moderate activity but ethanol extract of Terminalia chebula, proven to be having significant antioxidant activity over all extracts. Anti-inflammatory activity of MCF-7 breast cancer cell line exhibited that good activity with IC50 value 228.82µg/ml. Whereas Methanol extract of Terminalia bellirica and Aqueous extract of Phyllanthus emblica shown 432.45µg and 259.02µg respectively. Conclusion: The present study concludes that in among three tested extracts, Ethanol extract of Terminalia chebula exhibited prominent anticancer activity against Breast cancer (MCF-7) with significant IC50 value 228.82 µg. However further studies are needed to find out the structural of bioactive compounds and investigate mechanisms of antioxidant and anticancer activities of the bioactive compounds in in-vivo animal study.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.52711/0974-360X.2022.00490