Inhibitory Activity Of Essential Oils Of Curcuma Longa And Zingiber Officinale Against Burkholderia Glumae

Essential oils are organic compounds that during the last century have gained relevance in the pharmaceutical sector due to their antimicrobial properties, becoming viable alternative solutions for certain phytopathology. Rice plants are attacked by Burkholderia glumae, which causes bacterial blast....

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Bibliographic Details
Published in:Webology 2022-01, Vol.19 (6), p.262-272
Main Authors: Pérez-Cordero, Alexander, Canchila, Edin Arroyo, Montes- Vergara, Donicer E
Format: Article
Language:English
Subjects:
Agricultural production
Antimicrobial agents
Chromatography
Oils & fats
Pharmaceutical industry
Sensors
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Summary:Essential oils are organic compounds that during the last century have gained relevance in the pharmaceutical sector due to their antimicrobial properties, becoming viable alternative solutions for certain phytopathology. Rice plants are attacked by Burkholderia glumae, which causes bacterial blast. Therefore, the aim of this study was to evaluate in vitro the inhibitory activity of the essential oils of C. longa and Z. officinale on B. glumae. The plants were collected in a rural area in the municipality of Tolú Viejo-Sucre. Fresh rhizomes were washed with water and selected, then sliced and the essential oil was extracted from 1 kg of material from each species by the microwave-assisted hydrodistillation technique. The bacterial inoculum was mass seeded on KIN B agar plates. To evaluate inhibitory activity, the agar diffusion method was used using filter paper discs and DMSO as solvent. The dilutions of the oils were 1:1, 1:5 and 1:10 and 15 µl of each dilution was placed aseptically on the paper. The determination of the chemical components of the essential oils was carried out using the instrumental technique of Gas Chromatography with Mass Selective Detector (GC/MS). Five extractions of the essences were made from 200 g of sample, yielding an average of 0.50 g of oil per extraction from the rhizomes of C. longa, with a yield of 0.25 %, and 0.85 g of oil from the rhizomes of Z. officinale, with a yield of 0.43%. At 1:10 and 1:5 dilutions for the two oils there was no inhibitory activity and only at 1:1 dilution for the two oils there were inhibition halos showing slight inhibitory activity. There is inhibitory activity when the concentrations in the two types of oils exceed values of 500000 ppm, which suggests evaluating other variables that may be affecting the performance and efficiency of these oils to inhibit the growth of this phytopathogens.
ISSN:1735-188X