Highly sensitive detection of aflatoxin B1 by in situ growth of electroactive polymers via ring-opening metathesis polymerization

In this study, an electrochemical sensor was fabricated for detecting aflatoxin B1 (AFB1) based on in situ-initiated ring-opening metathesis polymerization. The thiol-modified aptamer was first immobilized on the Au electrode through Au-S bonding to capture AFB1. Then, the acrylic acid (AA)-function...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2022-12, Vol.372, p.132619, Article 132619
Main Authors: Yang, Huaixia, Liu, Zenghui, Zhou, Shijin, Cui, Xiaojing, Liu, Yanju
Format: Article
Language:English
Subjects:
Acrylic acid
Aflatoxins
Antibodies
Chemical sensors
Electroactive polymers
Electrodes
Metathesis
Polymerization
Ring opening
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Summary:In this study, an electrochemical sensor was fabricated for detecting aflatoxin B1 (AFB1) based on in situ-initiated ring-opening metathesis polymerization. The thiol-modified aptamer was first immobilized on the Au electrode through Au-S bonding to capture AFB1. Then, the acrylic acid (AA)-functionalized antibody specifically recognized AFB1 and immobilized on the surface of the electrode. Finally, a large number of cyclopentenyl ferrocene (CFc) monomers were grafted onto the electrode surface under the action of the catalyst AquaMet, forming an electroactive long-chain polymer that results in a significant signal enhancement. Under optimal conditions, the detection limit of the method was 0.34 fg/mL with a detection range of 2 fg/mL-2 ng/mL. The proposed sensor is highly selective due to the high specificity of the aptamer and antibody to the target. In addition, the sensor has been successfully applied to detect AFB1 in real samples with recoveries ranging from 92.91% to 107.20%.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2022.132619