Association of methylation status of ABCA1/G1 genes with the risk of coronary artery disease

• Published in: Egyptian Journal of Medical Human Genetics 2022-12, Vol.23 (1), p.167-10
• Main Authors: Mansouri, Elaheh, Esmaeili, Fataneh, Montaseri, Maryam, Emami, Mohammad Amin, Koochakkhani, Shabnaz, Khayatian, Mahmood, Zarei, Hasan
• Format: Article
• Language: English
• Subjects: ABCA1 protein; Apolipoproteins; Apolipoproteins ATP-binding cassette transporter; ATP-binding protein; Cardiovascular disease; Cardiovascular diseases; Cholesterol; Coronary artery disease; Coronary heart disease; Diabetes; Diabetes mellitus; Diabetes therapy; DNA methylation; Genes; Genetic research; Heart diseases; High density lipoprotein; Homeostasis; Medical imaging; Medical research; Medicine, Experimental; Methylation; Polymerase chain reaction; Risk factors
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1186/s43042-022-00381-y

Background ATP-binding cassette transporters A1/G1 (ABCA1/G1) is a main regulator of HDL (high-density lipoprotein) formation and reverse cholesterol transport. Impaired ABCA1/G1 genes function may seriously affect cholesterol homeostasis, leading to increased risk of cardiovascular disease. In the present study, the association of ABCA1/G1 genes methylation status with the risk of coronary artery disease (CAD), risk factors of CAD, and serum level of lipid parameters was investigated. This study was conducted on 70 CAD patients and 40 control subjects. All CAD subjects with diabetes mellitus were excluded. The promoter methylation status of ABCA1/G1 genes was determined by the methylation-specific polymerase chain reaction (MS-PCR) method and serum lipid parameters were assessed using commercial kits. Results ABCA1 promoter methylation was higher in CAD group compared to the control participants (80% vs. 60%). Hypermethylation of the ABCA1 gene significantly increases the risk of CAD in the total population (OR 3.886, 95% CI (1.181-12.791), p = 0.026). ABCG1 methylation status showed no difference between CAD and control subjects. In addition, no significant association was noted between methylation status of ABCA1/G1 and serum level of lipid profile. Conclusions Altogether, our study shows that ABCA1 gene promoter hypermethylation may increase the risk of CAD, which may help identify people at risk of developing CAD.