Network pharmacology-based analysis of the mechanism of Saposhnikovia divaricata for the treatment of type I allergy

Saposhnikovia divaricata (Turcz.) Schischk (Apiaceae) (SD) has various pharmacological activities, but its effects on type I allergy (TIA) have not been comprehensively studied. This study evaluates the treatment and molecular mechanisms of SD against TIA. The effective components and action targets...

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Bibliographic Details
Published in:Pharmaceutical biology 2022-12, Vol.60 (1), p.1224-1236
Main Authors: Li, Xiangsheng, Li, Hui, Wang, Tingting, Zhao, Yang, Shao, Yuxin, Sun, Yizhao, Zhang, Yanfen, Liu, Zhongcheng
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
allergic targets
Allergies
Arthritis
Calcium signalling
cell degranulation
Chinese medicine
Degranulation
Genes
IgE
Immunoglobulin E
Lyn protein
MAP kinase
Mast cells
Molecular modelling
Pharmaceuticals
Pharmacology
Research methodology
Science
Signal transduction
Syk protein
Traditional Chinese medicine
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Summary:Saposhnikovia divaricata (Turcz.) Schischk (Apiaceae) (SD) has various pharmacological activities, but its effects on type I allergy (TIA) have not been comprehensively studied. This study evaluates the treatment and molecular mechanisms of SD against TIA. The effective components and action targets of SD were screened using TCMSP database, and allergy-related targets of SD were predicted using GeneCards and OMIM database. The obtained target intersections were imported into David database for GO analysis, and used R software to perform KEGG analysis. The RBL-2H3 cells sensitised by DNP-IgE/DNP-BSA were treated with different concentrations of SD (root decoction, 0.5, 1, and 2 mg/mL), prim-O-glucosylcimifugin (POG, 10, 40, and 80 μg/mL) and the positive control drug-ketotifen fumarate (KF, 30 μM) for 12 h, then subjected to cell degranulation and qPCR analysis. Eighteen active compounds of SD and 38 intersection targets were obtained: TIA-related signal pathways mainly include calcium signal pathway, PI3K-Akt signal pathway and MAPK signal pathway. Taking the β-Hex release rate of the model group as the base, the release rate of SD and POG in high dose groups were 43.79% and 57.01%, respectively, which were significantly lower than model group (p 
ISSN:1388-0209
1744-5116
DOI:10.1080/13880209.2022.2086583