Improvement of irradiation-induced fibroblast damage by α2-macroglobulin through alleviating mitochondrial dysfunction

• Published in: Pharmaceutical biology 2022-12, Vol.60 (1), p.1365-1373
• Main Authors: Huangfu, Chaoji, Tang, Nan, Yang, Xiaokun, Gong, Zhanwei, Li, Junzheng, Jia, Junting, Zhang, Jingang, Huang, Yan, Ma, Yuyuan
• Format: Article
• Language: English
• Subjects: Apoptosis; Calcium (mitochondrial); Catalase; Cell growth; Cell migration; Cell proliferation; Enzyme-linked immunosorbent assay; Fibroblasts; Flow cytometry; G2 phase; Immunofluorescence; Membrane potential; Mitochondria; Oxidative stress; plasma product; plasma protein; proteinase inhibitor; Radiation; S phase; Superoxide dismutase; Transient receptor potential proteins; TRPM2; Western blotting; Wound healing
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880209.2022.2096077

α2-Macroglobulin (α2-M) is believed to be a potential anti-irradiation agent, but related mechanisms remains unclear. We investigated the irradiation protective effect of α2-M. A total of 10 Gy dose of irradiation was used to damage human skin fibroblasts. The influence of α2-M (100 µg/mL) on the proliferation, migration, invasion and apoptosis of fibroblasts was observed using Cell Counting Kit-8 (CCK8), wound healing, transwell, and flow cytometry. Malondialdehyde, superoxide dismutase and catalase was measured using related ELISA kits. The levels of mitochondrial membrane potential and calcium were detected using flow cytometry. The expression of transient receptor potential melastatin 2 (TRPM2) was investigated through western blotting and immunofluorescence staining. High purity of α2-M was isolated from Cohn fraction IV. α2-M significantly increased cell proliferation, migration, invasion, but suppressed cell apoptosis after irradiation. The promotion of cell proliferation, migration and invasion by α2-M exceeded over 50% compared group irradiation. The increased cell ratio in the S phase and decreased cell ratio in the G2 phase induced by irradiation were remarkably reversed by α2-M. α2-M markedly suppressed the increased oxidative stress level caused by irradiation. The mitochondrial damage induced by irradiation was improved by α2-M through inhibiting mitochondrial membrane potential loss, calcium and TRPM2 expression. α2-M significantly promoted the decreased fibroblast viability and improved the mitochondria dysfunction caused by irradiation. α2-M might present anti-radiation effect through alleviating mitochondrial dysfunction caused by irradiation. This study could provide a novel understanding about the improvement of α2-M on irradiation-induced injury.