Characterization of Silver Ions-Doped Organomodified Nanoclays

• Published in: Arabian journal for science and engineering (2011) 2023, Vol.48 (1), p.327-340
• Main Authors: İlaslan, Kerem, Tornuk, Fatih
• Format: Article
• Language: English
• Subjects: Bacteria; Cations; Cetyltrimethylammonium bromide; Dilution; E coli; Engineering; Humanities and Social Sciences; Interlayers; Montmorillonite; multidisciplinary; Research Article-Chemistry; Science; Silver; Surfactants; Zeta potential
• ISSN: 2193-567X; 1319-8025; 2191-4281
• DOI: 10.1007/s13369-022-07046-3

In this study, montmorillonite (MMT) and halloysite nanoclays were organomodified with cationic surfactants N-cetyl-N, N, N-trimethylammonium bromide (CTAB) and poly diallyl dimethylammonium chloride (PDAC) to enhance intercalation characteristics. The organomodified nanoclay samples were doped with Ag + ions in order to enhance antimicrobial properties, and their XRD, ATR-FTIR, ICP-MS, SEM, TEM, zeta potential, mean particle size, and in vitro Ag + release properties were further investigated. The antibacterial activity of the Ag-doped organoclays was analyzed by broth dilution method as well as the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive ( Staphylococcus aureus ATCC 25,923 and Listeria monocytogenes ATCC 13,932) and gram-negative ( Escherichia coli O157:H7 ATCC 25,922 and Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14,028) bacterial strains. The surface load of the organomodified nanoclays changed to positive due to the cationic surfactants, and as a result of the XRD examinations, the interlayer space of the nanoclays increased. ATR-FTIR and ICP-MS analysis indicated that Ag + ions successfully doped into the nanoclay structure. Also, MMT-type nanoclay samples released the Ag + ions into the water medium more than halloysite-type nanoclay samples. Nanoclays organomodified by CTAB had an effective bactericidal effect on each bacterial strain than PDAC-modified nanoclays. Ag-doped nanoclays had MIC and MBC values varying from 0.6 to 5 mg/ml in the nutrient broth medium for all the bacterial strains. In conclusion, intercalated and Ag + -doped MMT and halloysite nanoclays were successfully prepared and effectively used for bacterial growth inhibition.