Azure A embedded in carbon dots as NADH electrocatalyst: Development of a glutamate electrochemical biosensor

Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. A deep characterization of AA-CDs nanomaterial has been carried out, proving the covalent attachment of azure A molecules into the carbon dots nanostructure. Disposabl...

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Published in:Sensors and actuators. B, Chemical Chemical, 2023-01, Vol.374, p.132761, Article 132761
Main Authors: Martínez-Periñán, Emiliano, Domínguez-Saldaña, Aitor, Villa-Manso, Ana M., Gutiérrez-Sánchez, Cristina, Revenga-Parra, Mónica, Mateo-Martí, Eva, Pariente, Félix, Lorenzo, Encarnación
Format: Article
Language:English
Subjects:
Azure A
Biological properties
Biosensors
Carbon
Carbon dots
Carbon nanodot
Chitosan
Electrocatalysts
Electrodes
Glutamate Biosensor
Glutamate dehydrogenase
NADH
Nanomaterials
Nicotinamide adenine dinucleotide
Oxidation
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Summary:Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. A deep characterization of AA-CDs nanomaterial has been carried out, proving the covalent attachment of azure A molecules into the carbon dots nanostructure. Disposable screen-printed carbon electrodes (SPCE) have been modified with AA-CDs, through the action of chitosan polymer (Chit-AA-CDs/SPCE). The Chit-AA-CDs/SPCE electrocatalytic activity towards the oxidation of NADH has been proved, obtaining excellent results regarding the low oxidation potential achieved (−0.15 V vs. Ag) and low detection and quantification limits (LOD and LOQ) for NADH, 16 and 53 µM, respectively. The developed electrochemical platform has been applied for the construction of a glutamate biosensor by immobilizing L-glutamic dehydrogenase (GLDH/Chit-AA-CDs/SPCE). The morphology of GLDH/Chit-AA-CDs/SPCE platform was analysed by AFM at each different step of the electrode modification process. The resulting biosensing platform is capable of detect NADH enzymatically generated by GLDH in the presence of glutamate and NAD+. Good analytical parameters were obtained for glutamate analysis using GLDH/Chit-AA-CDs/SPCE, as LOD and LOQ of 3.3 and 11 µM, respectively. The biosensor has been successfully applied to the analysis of food and biological samples. •Azure A modified Carbon nanodots (AA-CDs) as electrocatalyst for NADH oxidation.•A disposable enzymatic electrochemical biosensor for glutamate determination.•Phenothiazine covalent insertion in carbon nanodot structure.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2022.132761