Simultaneous Determination of Multiple Components in Jinshui Huanxian Granules by UPLC–Orbitrap Fusion MS

Jinshui Huanxian granules (JSHX) is a clinical Chinese medicine formula used for the treatment of pulmonary fibrosis (PF). In this study, an accurate method for the simultaneous determination of 37 components in JSHX was established based on ultra-high-performance liquid chromatography coupled with...

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Bibliographic Details
Published in:Chromatographia 2023, Vol.86 (1), p.21-30
Main Authors: Yuan, Jie, Liu, Xue-Fang, Zhao, Di, Tian, Yan-Ge, Zhang, Hao-Jie, Cui, Hong-xin, Feng, Su-Xiang
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aqueous solutions
Chemistry
Chemistry and Materials Science
Chromatography
Flow velocity
Formic acid
Granular materials
High performance liquid chromatography
Ions
Laboratory Medicine
Mass spectrometry
Negative ions
Original
Pharmacy
Proteomics
Quality assessment
Scientific imaging
Spectroscopy
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Summary:Jinshui Huanxian granules (JSHX) is a clinical Chinese medicine formula used for the treatment of pulmonary fibrosis (PF). In this study, an accurate method for the simultaneous determination of 37 components in JSHX was established based on ultra-high-performance liquid chromatography coupled with Orbitrap Fusion mass spectrometry (UPLC–Orbitrap Fusion MS). Chromatographic separation was performed on Accucore TM C18 column (2.1 mm × 100 mm, 2.6 μm) using methanol–0.1% formic acid aqueous solution gradient elution program at a flow rate of 0.2 mL/min with a total running time of 60 min. The analytes were ionized by electrospray ionization in the both positive and negative ion mode, and detected by Orbitrap Fusion mass spectrometry. Regression equations of all the components showed excellent linearity ( r  ≥ 0.9990) in the concentration range, the precision, repeatability and stability (relative standard deviations, RSD) for all components were less than 3%, and the mean recoveries of the 37 components were ranged from 96.91% to 101.67%. Using UPLC–Orbitrap Fusion MS, an accurate, simple, and reliable method for the simultaneous determination of 37 components in JSHX was first developed and validated, which provides a scientific basis for the quality evaluation and control of Jinshui Huanxian granules. Graphical Abstract
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-022-04213-4