Investigation of antihyperalgesic and antiedematous activities of three Hieracium species

• Published in: Natural product research 2021-12, Vol.35 (23), p.5384-5388
• Main Authors: Milutinović, Violeta, Pecikoza, Uroš, Tomić, Maja, Stepanović-Petrović, Radica, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana
• Format: Article
• Language: English
• Subjects: Acetates; Acetic acid; acute toxicity screening; antiedematous activity; antihyperalgesic activity; Carrageenan; carrageenan-induced inflammation; Dichloromethane; Edema; Flowering; Hieracium; Toxicity; triterpene/polyphenol rich extracts
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2020.1768086

Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50-200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%-56.2% (ED 50 =163.0 ± 26.5 mg/kg) and 25.3%-51.6% (ED 50 =211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.