Effect of different lignocellulosic biomasses on laccase production by Pleurotus species

• Published in: Bioresources 2022-08, Vol.17 (3), p.4921-4936
• Main Authors: Han, Mei-Ling, Li, Xue-Qing, Zhang, Chun-Dan, Li, Mei-Xue, Zhang, Meng-Han, An, Min, Dou, Xin-Ying, Zhang, Tian-Xin, Yan, Xun-You, Bian, Lu-Sen, An, Qi
• Format: Article
• Language: English
• Subjects: Biomass; Energy consumption; Enzymes; Fermentation; Fungi; Hardwoods; Industrial applications; Laccase; Lignin; Lignocellulose; Low cost; Microorganisms; Pleurotus; Softwoods; Solid state; Solid state fermentation; Sophora japonica; Substrates
• ISSN: 1930-2126; 1930-2126
• DOI: 10.15376/biores.17.3.4921-4936

The laccase activity of three cultivated Pleurotus strains was investigated using different lignocellulosic biomasses for solid-state fermentation. The lignocellulosic biomasses were Sophora japonica, Salix babylonica, Populus beijingensis, and Pinus tabuliformis, which were selected because of their laccase producing ability and low cost. The maximum laccase activities from P. ostreatus Han 1189, P. citrinopileatus Han 1192, and P. eryngii Han 1205 using Sophora japonica were 768.74 U/L ± 11.25 U/L, 144.77 U/L ± 9.70 U/L, and 200.42 U/L ± 6.85 U/L, respectively. The three species of Pleurotus showed consistency in having a preference of hardwood to secret laccase under solid-state fermentation when facing hardwood and softwood as substrates. Furthermore, the presence of Sophora japonica was beneficial to improving the laccase activity for three Pleurotus strains. The capacity of the laccase secretion of P. ostreatus Han 1189 was superior to the capacity of P. citrinopileatus Han 1192 and P. eryngii Han 1205. The discovery was helpful for obtaining new high-producing strains and suitable wood materials, as well as expanding these high-producing strains for industrial applications to obtain high laccase activity, high laccase yield, and low cost laccase.