Diagnostic, prognostic, and immunological roles of CD177 in cervical cancer

• Published in: Journal of cancer research and clinical oncology 2023, Vol.149 (1), p.173-189
• Main Authors: Liao, Wenxin, Li, Wei, Li, Yang, Liu, Tingting, Wang, Ying, Feng, Dan, Shen, Fujin
• Format: Article
• Language: English
• Subjects: Bioinformatics; Biomarkers; Blotting, Western; Cancer Research; CD27 antigen; CD80 antigen; Cervical cancer; CTLA-4 protein; Female; Gefitinib; Gene set enrichment analysis; GPI-Linked Proteins - metabolism; Hematology; Humans; Immune checkpoint; Immune response; Immunology; Infiltration; Internal Medicine; Isoantigens - metabolism; Medical prognosis; Medicine; Medicine & Public Health; Metastases; Nomograms; Oncology; Prognosis; Receptors, Cell Surface - metabolism; Tumor cells; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - immunology
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-022-04465-5

Background CD177, an indicator of prognosis in diverse cancers, is involved in the physiological processes of various tumor cells, and acts as an immune molecule with novel functions in cancer pathogenesis. However, the diagnostic, prognostic, and immunological role of CD177 in cervical cancer remains unclear. Methods Utilizing publicly available databases and integrating several bioinformatics analysis methods, we evaluated the expression level of CD177 in cervical cancer by GENT2, HPA, and GEO databases. And the experiments of western blot and immunohistochemical staining were used to test the hypothesis. The Kaplan–Meier Plotter database, Xena Shiny, and the constructed nomogram were clearly demonstrated its prognostic value for patients. Gene set enrichment analysis explored the relationship between CD177 and cervical cancer immune responses and immune cells infiltration level. In addition, we investigated the association between CD177 expression and stromal score, immune score, immune checkpoint, and drug sensitivity by TCGA RNA-seq data. Results CD177 was apparently expressed at low levels in cervical cancer and predicted a poor survival rate for patients. CD177 significantly activated immune-related signaling pathways and had a positive relationship with immune cell infiltration level. The high CD177 expression group possessed the high stromal score and immune score. CD177 had potential interactions with CTLA4, CD27, BLTA, CD200R1, CD80, NRP1, TNFRSF25, TIGIT, ICOS, and TNFSF9 checkpoint markers. And CD177 expression was positively relevant with drug sensitivity for Lapatinib, Belinostat, ATRA, Gefitinib, Navitoclax, and Tamoxifen. Significance These findings may shed light on the vital role of CD177 in cervical cancer diagnosis, prognosis, and immunological functions, and it may be a promising predictor and potential factor for cervical cancer patients.