Nanoarchitectonics of electrochemical aptasensor based on electrospun carbon nanofibers and gold nanoparticles for tetracycline detection in chicken ham

• Published in: Applied physics. A, Materials science & processing Materials science & processing, 2023-03, Vol.129 (3), Article 176
• Main Authors: Mohammadi, Somayeh, Pooshang Bagheri, Kamran, Mousavi Nadushan, Rezvan, Adabi, Mahdi
• Format: Article
• Language: English
• Subjects: Applied physics; Biosensors; Carbon fibers; Characterization and Evaluation of Materials; Condensed Matter Physics; Electrodes; Heat treatment; Machines; Manufacturing; Materials science; Nanofibers; Nanoparticles; Nanotechnology; Optical and Electronic Materials; Physics; Physics and Astronomy; Polyacrylonitrile; Processes; Raman spectroscopy; Reproducibility; Spectrum analysis; Surfaces and Interfaces; Thin Films
• ISSN: 0947-8396; 1432-0630
• DOI: 10.1007/s00339-023-06416-4

An electrochemical aptamer-based sensor was designed to detect tetracycline (Tet) in chicken ham. First, polyacrylonitrile (PAN) polymer was used to prepare electrospun PAN nanofibers. PAN nanofibers were heat treated to form electrospun carbon nanofiber (CNF) mat. The electrospun CNF mat electrode was modified with Au and the aptamer of Tet was placed on the mat electrode. Raman spectroscopy and scanning electron microscopy equipped with energy-dispersive spectroscopy were used to identify the structure and morphology of electrospun CNFs, respectively. The behavior of CNF mat electrode was evaluated via cyclic voltammetry. The designed aptamer biosensor had a good repeatability, reproducibility, stability, and specific function for the detection of Tet in chicken ham. This biosensor was able to detect the Tet in the linear range of 1 × 10 –9  M to 1 × 10 –4  M with the detection limit of 1.2 × 10 –10  M and preserve about 95% of its primary response after 20 days. In addition, the recoveries of Tet were achieved ranging from 96.92 to 104.31%.