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Biocontrol potential of Bacillus licheniformis W10 against peach shoot blight caused by Phomopsis amygdali

Peach shoot blight caused by Phomopsis amygdali has led to considerable economic losses in major peach fruit-growing areas. A biocontrol bacterium, Bacillus licheniformis W10 was successfully identified in our previous study. Furthermore, we found that B. licheniformis W10 could produce an extracell...

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Published in:European journal of plant pathology 2023-03, Vol.165 (3), p.559-567
Main Authors: Ji, Zhaolin, Liu, Yang, Gao, Rujia, Zhang, Liang, Zhu, Feng, Yang, Lina, Dong, Jingping, Xu, Jingyou
Format: Article
Language:English
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Summary:Peach shoot blight caused by Phomopsis amygdali has led to considerable economic losses in major peach fruit-growing areas. A biocontrol bacterium, Bacillus licheniformis W10 was successfully identified in our previous study. Furthermore, we found that B. licheniformis W10 could produce an extracellular antifungal protein that exhibited a strong inhibitory effect against many plant pathogens. However, the biocontrol potential of B. licheniformis W10 against peach shoot blight pathogen is still unclear. Therefore, the biocontrol effects of the B. licheniformis W10 bacterial suspension and its antifungal protein on  P. amygdali were investigated. The results showed that the B. licheniformis W10 bacterial suspension and antifungal protein inhibit mycelial growth, sporulation, conidial germination and germ-tube elongation of P. amygdali . The bacterial suspension (2 × 10 9  cfu/mL) and antifungal protein at 1.5320 mg/mL completely inhibited mycelial growth. A 5 × 10 9  cfu/mL bacterial suspension and antifungal protein at 1.5320 mg/mL had 94.9% and 87.5% inhibition rates on the sporulation of P. amygdali , respectively. The bacterial suspension of 1 × 10 9  cfu/mL and antifungal protein at 6.4992 mg/mL completely inhibited the conidial germination and germ-tube elongation of P. amygdali . Furthermore, low concentrations of the bacterial suspension (5 × 10 8  cfu/mL) and antifungal protein (1.6248 mg/mL) still had an above 80% inhibition rate against conidial germination and germ-tube elongation. In addition, the bacterial suspension and antifungal protein were demonstrated to have destructive effects on the hyphal shape and structure of P. amygdali , with hyphal cell distortion, tumescence, protoplasm leakage, cell wall breakage, and hyphal rupture. Overall, our results suggest that B. licheniformis W10 can be used as a potential biocontrol agent for the management of peach shoot blight.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-022-02627-2