Martynia annua safety and efficacy: heavy metal profile, in silico and in vitro approaches on antibacterial and antidiabetic activities

• Published in: Natural product research 2023-03, Vol.37 (6), p.1016-1022
• Main Authors: Alrabie, Ali, Al-Rabie, Nabeel Abdullah, Al Saeedy, Mohammed, Al Adhreai, Arwa, Al-Qadsy, Inas, Farooqui, Mazahar
• Format: Article
• Language: English
• Subjects: alpha-Amylases; alpha-Glucosidases; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Cadmium; Diabetes mellitus; E coli; Escherichia coli; Glucosidase; Heavy metals; Hypoglycemic Agents - pharmacology; Lead; Liquid chromatography; Martynia annua; Mass spectrometry; Mass spectroscopy; Metals, Heavy - analysis; Molecular docking; Molecular Docking Simulation; Plant Extracts - chemistry; Safety; Seeds; α-Amylase; α-Glucosidase
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2097227

Liquid Chromatography-Mass Spectrometry (LC-MS) analysis of methanol extract of seed revealed the presence of haploperozide and austricine. For safety, heavy metals content investigation of plant powder using the Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) technique showed that the toxic metals (Pb: 2.07 mg/kg; Cd: 0.07 mg/kg; and As: 0.18 mg/kg) concentrations were found to be below the permissible limit. The extract demonstrated significant antibacterial activity against (MIC value 125 g/mL). Furthermore, it was effective in inhibiting both α-glucosidase and α-amylase enzymes with a high percentage and IC values were 42.28 ± 0.39 µg/mL and 34.11 ± 0.31 µg/mL, respectively. These findings were supported by a molecular docking study, some of the phytochemicals showed higher docking score values than references. However, seeds are safe to consume because they contain low levels of toxic heavy metals and possess antibacterial and anti-diabetic properties.