Multiple Solid–Liquid Extraction: Determination of Extraction Yield through UV–Vis Spectroscopy

A class of 18 students attending the last year of an Italian high school was divided into groups and performed the extraction of pigments contained in hibiscus tea. Each group worked at a different temperature and carried out multiple extractions using distilled water as a solvent. By means of UV–vi...

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Bibliographic Details
Published in:Journal of chemical education 2023-02, Vol.100 (2), p.875-879
Main Authors: Bergamin, Elia, Fabris, Giacomo, Neffat, Matteo, Toaldo, Paola Baron, Luchi, Daniela De, Bonomi, Renato
Format: Article
Language:English
Subjects:
Data processing
Distilled water
Educational attainment
Extraction processes
Pigments
Reagents
Secondary education
Spectroscopy
Spectrum analysis
Students
Temperature effects
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Summary:A class of 18 students attending the last year of an Italian high school was divided into groups and performed the extraction of pigments contained in hibiscus tea. Each group worked at a different temperature and carried out multiple extractions using distilled water as a solvent. By means of UV–vis spectroscopy it was possible to determine the yield of each individual extraction and then to study the effect of temperature on the extraction itself. The method is easy to implement, it does not require the use of hazardous reagents or special safety precautions, and it is very cheap. The laboratory part was carried out in 3 h time, while the analysis and data processing took about 4 h. On an educational level, the students learned to process and analyze large amounts of data, and they became familiar with the UV–vis spectrophotometer and eventually had to solve the problems independently.
ISSN:0021-9584
1938-1328
DOI:10.1021/acs.jchemed.2c00914