The Use of Phage Antibodies for Microbial Cell Detection

Phage antibody display technology has revolutionized the field of bacterial immunodetection. This technology allows the expression of an antibody fused to the coat protein of a filamentous bacteriophage. The use of phage display makes it possible to obtain high-affinity antibodies, bypassing the sta...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2022-12, Vol.58 (Suppl 1), p.S32-S46
Main Authors: Guliy, O. I., Evstigneeva, S. S., Dykman, L. A.
Format: Article
Language:English
Subjects:
Antibodies
Biochemistry
Biomedical and Life Sciences
Biosensors
Coat protein
Display devices
Immunization
Life Sciences
Medical Microbiology
Microbiology
Microorganisms
Phage display
Phages
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Summary:Phage antibody display technology has revolutionized the field of bacterial immunodetection. This technology allows the expression of an antibody fused to the coat protein of a filamentous bacteriophage. The use of phage display makes it possible to obtain high-affinity antibodies, bypassing the stage of animal immunization, reducing the time for obtaining stable antibody-producing clones from several months to several weeks, and significantly reducing the cost of the process. These advantages make phage antibodies an important tool for bacterial detection. This paper presents a brief description of the technological methods for obtaining phage antibodies to microbial cells. The possibilities and prospects for using phage antibodies as a selective agent in analytical systems, including biosensors, are discussed.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683822100076