Anticancer, anti-biofilm and antimicrobial activity of fucoidan-loaded zeolitic imidazole framework fabricated by one-pot synthesis method

• Published in: Applied nanoscience 2023, Vol.13 (3), p.1919-1937
• Main Authors: Raju, Prabhu, Natarajan, Suganthy
• Format: Article
• Language: English
• Subjects: Anticancer properties; Antiinfectives and antibacterials; Antimicrobial agents; Biocompatibility; Biofilms; Biomedical materials; Cancer; Chemistry and Materials Science; Drug delivery systems; E coli; Imidazole; Klebsiella; Lethality; Materials Science; Membrane Biology; Nanochemistry; Nanotechnology; Nanotechnology and Microengineering; Original Article; Particle size distribution; Porous materials; Staphylococcus infections; Sustained release; Synthesis; Two dimensional analysis
• ISSN: 2190-5509; 2190-5517
• DOI: 10.1007/s13204-021-01881-w

Biocompatible drug delivery system with precise and sustained release is necessary for biomedical applications. Zeolitic imidazole frameworks (ZIF-L), an emerging porous material, have been widely reported as efficient platform for the drug delivery. The present study focuses on one-pot organic solvent-free synthesis of fucoidan-loaded ZIF-L (FU@ZIF-L) and assess its biomedical applications. Antimicrobial, anticancer efficiency and biocompatibility of FU@ZIF-L was systematically investigated. Formation of FU@ZIF-L was confirmed by UV–Visible spectroscopy, while XRD analysis revealed presence of two-dimensional flaky crystalline structures, which was substantiated by TEM analysis. DLS analysis showed homogenously distributed crystals with particle size of 78 nm suitable for cellular intake. Results revealed that FU@ZIF-L exhibited potent antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia and Escherichia coli. In addition, FU@ZIF-L was observed to be more efficient in piercing and disturbing the biofilm architecture of methicillin-resistant Staphylococcus aureus and E. coli . Furthermore, FU@ZIF-L exhibited cytotoxic effect against A549 cells with IC 50 value of 38.5 ± 2.34 μg/ml. ROS-mediated nuclear damage might be the reason for its anticancer potential. Brine shrimp lethality assay confirmed the biocompatible nature of FU@ZIF-L. To conclude, ZIF-L acts as effective drug delivery system for fucoidan enhancing its biomedical applications.