Genome-derived Microsatellite Markers in Lippia origanoides from Colombia

Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from...

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Bibliographic Details
Published in:Journal of herbs, spices & medicinal plants spices & medicinal plants, 2023-04, Vol.29 (2), p.168-185
Main Authors: Santamaría-Acevedo, Liliana, Levy, María Mercedes, Torres-Martínez, Lorena, Marchant, Sergio, Rondón-González, Fernando, Stashenko, Elena E., Levy, Morris, Fuentes, Jorge Luis
Format: Article
Language:English
Subjects:
Flowers & plants
Gene sequencing
Genetic analysis
Genetic markers
Genetic resources
Genomes
Genomics
Lippia
Lippia origanoides
microsatellite
Microsatellites
Municipalities
population genetic analysis
Population genetics
Verbenaceae
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Summary:Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.
ISSN:1049-6475
1540-3580
DOI:10.1080/10496475.2022.2121351