Genome-derived Microsatellite Markers in Lippia origanoides from Colombia

Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from...

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Published in:Journal of herbs, spices & medicinal plants spices & medicinal plants, 2023-04, Vol.29 (2), p.168-185
Main Authors: Santamaría-Acevedo, Liliana, Levy, María Mercedes, Torres-Martínez, Lorena, Marchant, Sergio, Rondón-González, Fernando, Stashenko, Elena E., Levy, Morris, Fuentes, Jorge Luis
Format: Article
Language:English
Subjects:
Flowers & plants
Gene sequencing
Genetic analysis
Genetic markers
Genetic resources
Genomes
Genomics
Lippia
Lippia origanoides
microsatellite
Microsatellites
Municipalities
population genetic analysis
Population genetics
Verbenaceae
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container_end_page 185
container_issue 2
container_start_page 168
container_title Journal of herbs, spices & medicinal plants
container_volume 29
creator Santamaría-Acevedo, Liliana
Levy, María Mercedes
Torres-Martínez, Lorena
Marchant, Sergio
Rondón-González, Fernando
Stashenko, Elena E.
Levy, Morris
Fuentes, Jorge Luis
description Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.
doi_str_mv 10.1080/10496475.2022.2121351
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subjects Flowers & plants
Gene sequencing
Genetic analysis
Genetic markers
Genetic resources
Genomes
Genomics
Lippia
Lippia origanoides
microsatellite
Microsatellites
Municipalities
population genetic analysis
Population genetics
Verbenaceae
title Genome-derived Microsatellite Markers in Lippia origanoides from Colombia
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