Clear evidence of the carcinogenic potential of anthracene: A 2‐year feeding study in rats and mice

• Published in: Environmental toxicology 2023-03, Vol.38 (4), p.709-726
• Main Authors: Takeda, Tomoki, Suzuki, Masaaki, Kano, Hirokazu, Matsumoto, Michiharu, Umeda, Yumi
• Format: Article
• Language: English
• Subjects: Adenoma; adverse effects; Animals; Anthracene; anthracenes; BDF1 mouse; Bioaccumulation; Bladder; Body weight; Cancer; Carcinogenesis; Carcinogenicity; Carcinogenicity Tests; Carcinogens; Carcinogens - toxicity; Chronic toxicity; compliance; Diet; ecotoxicology; Endometrium; F344 rat; Female; Females; Fibroadenoma; good laboratory practices; guidelines; Hepatocellular carcinoma; hepatoma; human health; Humans; Kidney cancer; Kidney Neoplasms - pathology; Lesions; Liver cancer; Liver Neoplasms - pathology; Male; Males; Mammary gland; Mammary glands; Mice; Mice, Inbred Strains; Neoplasms; Oral administration; Papilloma; pollutants; Rats; Rats, Inbred F344; Renal cell carcinoma; Sarcoma; Side effects; Survival; survival rate; Toxicity; Transitional cell carcinoma; Tumors; Urinary bladder
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.23722

Anthracene is an environmental pollutant and its adverse effects on human health have long been a concern due to its persistence and bioaccumulation properties. However, there is insufficient evidence for its chronic toxicity, especially carcinogenicity, in either humans or experimental animals. In this study, its carcinogenicity and chronic toxicity were investigated in compliance with the OECD test guideline 451 (OECD TG 451) and Good Laboratory Practice Standards. Fifty male and 50 female F344 rats and 50 female BDF1 mice were administrated 0, 8000, 20000, or 50000 ppm anthracene in the diet for 104 weeks, and 50 male BDF1 mice were fed diets containing anthracene at 0, 3200, 8000, or 20000 ppm. Anthracene treatment had no adverse effect on either the survival rate or general condition of the rats or mice during the study period. Body weights were lower or tended to be lower in the anthracene‐treated groups than in the control groups. Increased incidence of hepatocellular carcinoma and hepatocellular adenoma was observed in male rats and female mice. Renal cell carcinoma and renal cell adenoma, fibroadenoma in the mammary gland, and uterine endometrial stromal sarcoma were increased in female rats. Transitional cell carcinoma and transitional cell papilloma in the urinary bladder were also increased in male and female rats. In addition, several different pre‐neoplastic lesions were increased in the anthracene‐treated male and female rats and female mice. These results provide clear evidence that oral administration of anthracene for 104 weeks has a carcinogenic effect in male and female rats and female mice.