Involvement of Acetylcholine and Na+,K+-ATPase in the Regulation of Skeletal Muscle Growth in a Chicken Embryo

Recently, much attention has been paid to the discussion of the trophic function of the nervous system and its involvement in triggering the signaling cascades that regulate cellular metabolism. The role of acetylcholine and Na + ,K + -ATPase in the regulation of skeletal muscle growth was evaluated...

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Bibliographic Details
Published in:Journal of evolutionary biochemistry and physiology 2023, Vol.59 (1), p.285-292
Main Authors: Lopatina, E. V., Gavrichenko, A. V., Pasatetskaya, N. A.
Format: Article
Language:English
Subjects:
Acetylcholine receptors (nicotinic)
Animal Physiology
Biochemistry
Biomedical and Life Sciences
Cell culture
Embryos
Evolutionary Biology
Experimental Papers
Explants
Life Sciences
Na+/K+-exchanging ATPase
Nervous system
Ouabain
Skeletal muscle
Tissue culture
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Summary:Recently, much attention has been paid to the discussion of the trophic function of the nervous system and its involvement in triggering the signaling cascades that regulate cellular metabolism. The role of acetylcholine and Na + ,K + -ATPase in the regulation of skeletal muscle growth was evaluated in 10–12-day-old chicken embryos under conditions of organotypic tissue culture. Acetylcholine had a maximum trophotropic effect at a concentration 10 –8 M. An inhibitory analysis proved the involvement of the nicotinic acetylcholine receptor (nAChR) in this effect. Ouabain regulated the growth of skeletal muscle explants in a dose-dependent manner. At concentrations comparable to the physiological, it stimulated the growth of experimental explants by 33% compared to the control value. Ouabain was found to have myotoxic properties in the concentration range of 10 –6 –10 –4 M. Acetylcholine abolished the myotoxic effect of ouabain (10 –6 M) both directly, acting on Na + ,K + -ATPase, and via the nAChR.
ISSN:0022-0930
1608-3202
DOI:10.1134/S0022093023010234