Characterization ofAnabaena cylindrica solution system using synchronous-scan fluorescence spectroscopy

• Published in: Wuhan University journal of natural sciences 2005-03, Vol.10 (2), p.441-447
• Main Authors: Xian-li, Liu, nan-sheng, Deng, Shu, Tao
• Format: Article
• Language: English
• Subjects: Algae; Aqueous solutions; Emission analysis; Emissions; Fluorescence; Fluorescence spectroscopy; Iron; Quenching; Spectroscopy; Wavelength
• ISSN: 1007-1202; 1993-4998
• DOI: 10.1007/BF02830684

The characterization of the algaeAnabaena cylindrica solution with Fe (III) was investigated using fluorescence emission and synchronous-scan spectroscopy. The ranges of concentrations of algae and Fe (III) in aqueous solutions were 5.0×108 2.5×108 cell/L and 1060 μmol/L. respectively. The effective characterization method used was synchronous-scan fluorescence spectroscopy (SFS). The wavelength difference (Δλ) of 90 nm, was maintained between excitation wavelength (λen) and emission wavelength (λen). The peak was observed at about (λex) (λem) 326 nm for synchronous scan fluorescence spectroscopy. The fluorescence quenching in system of algae Fe(III)-HA was studied using synchronous-scan spectroscopy for the first time, Fe(III) was clearly the effective quencher. The relationship betweenI I (quenching efficiency) andc (concentration of Fe (III) added) was, a linear correlation for the algae solution with Fe(III). Also, Aldrich humie acid (HA) was found to be an effective quencher.