Production of lyophilized culture ofLactobacillus acidophilus with preserving cell viability

Optimal lyophilization process was developed for manufacturing the dried product ofLactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of a...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 1999-04, Vol.4 (1), p.36-40
Main Authors: Kang, Moo Heon, Saraswat, Vibhor, Lee, Jeewon, Park, Young-Hoon
Format: Article
Language:English
Subjects:
Biomass
Calcium chloride
Cell culture
Cell death
Cell survival
Cell viability
Colloiding
Freeze drying
Freezing
Glycerol
manufacturing
Methods
Phase transitions
Process parameters
Skim milk
Specific surface
Surface area
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Summary:Optimal lyophilization process was developed for manufacturing the dried product ofLactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above: an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm⁻¹ specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk+1% glycerol +0.1% calcium chloride.
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02931911