Characterization of the α-mannosidase gene family in filamentous fungi: N-glycan remodelling for the development of eukaryotic expression systems

Although filamentous fungi are used extensively for protein expression, their use for the production of heterologous glycoproteins is constrained by the types of N-glycan structures produced by filamentous fungi as compared to those naturally found on the glycoproteins. Attempts are underway to engi...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2000-08, Vol.5 (4), p.227-233
Main Authors: Eades, C. Joshua, Hintz, William E.
Format: Article
Language:English
Subjects:
Eukaryotes
Evolution
Fungi
Genes
Genetic engineering
Genomes
Glycan
Glycoproteins
Mannosidase
Proteins
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Summary:Although filamentous fungi are used extensively for protein expression, their use for the production of heterologous glycoproteins is constrained by the types of N-glycan structures produced by filamentous fungi as compared to those naturally found on the glycoproteins. Attempts are underway to engineer the N-glycan synthetic pathways in filamentous fungi in order to produce fungal expression strains which can produce heterologous glycoproteins carrying specific N-glycan structures. To fully realize this goal, a detailed understanding of the genetic components of this pathway in filamentous fungi is required. In this review, we discuss the characterization of the α-mannosidase gene family in filamentous fungi and its implications for the elucidation of the N-glycan synthetic pathway.
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02942178